CD45 Antibody (2D-1) recommended for detection of CD45, CD45RO, CD45A, CD45B and all forms of LCA of mouse, rat and human origin by IP, IF, IHC(P) and FCM

CD45 Antibody (2D-1): sc-1187

CD45 Antibody (2D-1) is rated 4.4 out of 5 by 8.
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Datasheets

    • CD45 Antibody (2D-1) is a mouse monoclonal IgG1 κ, cited in 14 publications, provided at 200 µg/ml
    • Anti-CD45 Antibody (2D-1) is recommended for detection of CD45, CD45RO, CD45A, CD45B and all forms of LCA of mouse, rat and human origin by IP, IF, IHC(P) and FCM
    • Anti-CD45 Antibody (2D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to APC-Cy7 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (2D-1): sc-1187.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for CD45 Antibody (2D-1) for IHC(P) applications. These reagents are now offered in bundles with CD45 Antibody (2D-1) (see ordering information below).

Hello there. Do you guys do international delivery? I need CD45 Antibody (2D-1): sc-1187 (Saint Petersburg, Russia). What is your price if you do international delivery? How long it takes? And how long will take delivery to San Diego, CA (and price)?

Asked by: Nina Redfield
Thank you for your question. Please contact our Customer Care Department for further assistance.
Answered by: Technical Support
Date published: 2019-06-16

Is there any cd45 antibody can use in hamster?

Asked by: kaola2hao
Thank you for your question. Unfortunately, none of our CD45 antibodies are currently recommended for use in hamster, nor are we aware of any product citations using the CD45 antibodies in hamster. This monoclonal antibody, CD45 Antibody (B-8): sc-28369, is raised against a recombinant protein (amino acids 1075-1304 of CD45 of human origin) which shares 75% sequence identity with this published model hamster CD45 protein sequence, (accession number: XP_012971285). The exact epitope recognized by this monoclonal antibody has not been determined, however, based on this alignment there is a chance that the antibody may cross-react with hamster. However, because the alignment is not 100%, we still can not guarantee it.
Answered by: Technical Support
Date published: 2019-01-15

Can CD45 (2D-1): sc-1187 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (2D-1): sc-1187 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from not good detection of mouse CD45 cells mouse spleen staining shows less than 5% of positive cells.
Date published: 2018-05-12
Rated 4 out of 5 by from good antibody I got a free sample from the company a month ago, and it worked for my IHC-p slides and no background staining, etc.. The only reason why I am not giving a 5-stars is because the intensity of the staining was not very strong. I was trying to stain lymphocytes infiltration in human lung sections.
Date published: 2017-11-22
Rated 5 out of 5 by from Excellent antibody! The CD45 Antibody (2D-1): sc-1187 provides great data for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-01-18
Rated 5 out of 5 by from Superb FCM data of human peripheral blood Superb FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Produced excellent immunoperoxidase membrane Produced excellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Clean western blot using 293T cell lysates Clean western blot using 293T cell lysates. -SCBT Publication Review
Date published: 2015-03-03
Rated 5 out of 5 by from Published WB and IF data with Jurkat T cells Published WB and IF data with Jurkat T cells, K592 and primary T cells. -SCBT Publication Review
Date published: 2015-01-28
Rated 5 out of 5 by from Excellent Indirect FCM data of HuT 78 cells Excellent Indirect FCM data of HuT 78 cells stained with CD45 . -SCBT QC
Date published: 2013-07-03
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