CD45RA Antibody (4KB5) recommended for detection of CD45RA of human origin by WB, IP, IF, IHC(P) and FCM

CD45RA Antibody (4KB5): sc-20057

CD45RA Antibody (4KB5) is rated 5.0 out of 5 by 4.
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Datasheets

    • CD45RA Antibody (4KB5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • raised against human hairy cell leukaemia cells
    • recommended for detection of CD45RA of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM

Can CD45RA (4KB5): sc-20057 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45RA (4KB5): sc-20057 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in NAMALWA and GA-10 whole cell lysates. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Good WB publishable data gathered with human U266 Good WB publishable data gathered with human U266, ILKM2 and NOP2 cells. -SCBT Publication Review
Date published: 2015-03-26
Rated 5 out of 5 by from Superb immunoperoxidase membrane and cytoplasmic Superb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Produced superb FCM data for human peripheral Produced superb FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-08-21
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