CD45 Antibody (MB4B4) recommended for detection of CD45 of mouse origin by WB, IP, IF and FCM

CD45 Antibody (MB4B4): sc-21739

CD45 Antibody (MB4B4) is rated 4.2 out of 5 by 5.
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Datasheets

    • CD45 Antibody (MB4B4) is a rat monoclonal IgG, cited in 3 publications, provided at 200 µg/ml
    • raised against anti immunoglobulin activated mouse B lymphoblasts
    • recommended for detection of CD45 of mouse origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Hello guys. Can we use CD45 (MB4B4) for flow cytometry detection in Human Clinical Samples? Does it come pre-conjugated with PE or APC or FITC or any other? If no conjugation is available how should we use the same in a clinical setting? Please reply asap

Asked by: smit
Thank you for your question. Yes, this antibody is recommended for Flow Cytometry, however, we do not recommend nor warranty our products for clinical samples. This product is for research use only. We do not offer it conjugated with any of the conjugates, I would recommend another antibody against CD45 that does come conjugated: CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Alternatively, we can provide this mouse monoclonal antibody, CD45 conjugated with any of your desired conjugated, but this would be a special order. Please contact one of our Special Orders coordinators by email at [email protected] for more details.
Answered by: Tech Service
Date published: 2017-03-31

Can CD45 (MB4B4): sc-21739 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (MB4B4): sc-21739 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong band in WB in mouse cells at 1:500 dilution Strong band in WB in mouse cells at 1:500 dilution.
Date published: 2015-09-23
Rated 4 out of 5 by from Used for IP with CD4 T cells Used for IP with CD4 T cells. -SCBT QC
Date published: 2015-05-17
Rated 4 out of 5 by from Clean band observed at correct MW in western Clean band observed at correct MW in western blot analysis of CD45 expression in BYDP whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 4 out of 5 by from Successful pulldown of CD45 from murine T Successful pulldown of CD45 from murine T-cells and CD4 cells (also used to detect via immunoblot). -SCBT Publication Review
Date published: 2015-02-24
Rated 4 out of 5 by from Produced wonderful FCM data for C57BL/6 mouse Produced wonderful FCM data for C57BL/6 mouse splenocytes. -SCBT QC
Date published: 2013-05-19
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