CD45 Antibody (B-8) is a mouse monoclonal IgG2b κ, cited in 7 publications, provided at 200 µg/ml
raised against amino acids 1075-1304 of CD45 of human origin
CD45 Antibody (B-8) is recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and ELISA
Anti-CD45 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (B-8): sc-28369.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for CD45 Antibody (B-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with CD45 Antibody (B-8) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Technical Service 4
Date published: 2020-11-12
What is your recommended western blotting secondary antibody for use with CD45 (B-8): sc-28369?
Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Rated 5 out of
CD45 antibody specific for human!CD45 Antibody (B-8) is a mouse IgG that is a solid choice for both WB and IHC(P) applications. Specific for human, and does not cross-react with mouse CD45!
Date published: 2017-01-19
Rated 5 out of
Saw good staining in IFSaw good staining in IF.
Date published: 2015-04-28
Rated 5 out of
Produced superb immunoperoxidase membraneProduced superb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-04-07
Rated 5 out of
Robust signal observed at correct molecularRobust signal observed at correct molecular weight in western blot analysis of CD45 expression in Jurkat, BJAB and CTLL-2 whole cell lysates. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn