CD45 Antibody (B-8) recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and ELISA

CD45 Antibody (B-8): sc-28369

CD45 Antibody (B-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • CD45 Antibody (B-8) is a mouse monoclonal IgG2b κ, cited in 7 publications, provided at 200 µg/ml
    • raised against amino acids 1075-1304 of CD45 of human origin
    • CD45 Antibody (B-8) is recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CD45 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (B-8): sc-28369.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for CD45 Antibody (B-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with CD45 Antibody (B-8) (see ordering information below).

How I can QC CD45 

Asked by: Zuhoor
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Technical Service 4
Date published: 2020-11-12

What is your recommended western blotting secondary antibody for use with CD45 (B-8): sc-28369?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-02-04
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Rated 5 out of 5 by from CD45 antibody specific for human! CD45 Antibody (B-8) is a mouse IgG that is a solid choice for both WB and IHC(P) applications. Specific for human, and does not cross-react with mouse CD45!
Date published: 2017-01-19
Rated 5 out of 5 by from Saw good staining in IF Saw good staining in IF.
Date published: 2015-04-28
Rated 5 out of 5 by from Produced superb immunoperoxidase membrane Produced superb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Robust signal observed at correct molecular Robust signal observed at correct molecular weight in western blot analysis of CD45 expression in Jurkat, BJAB and CTLL-2 whole cell lysates. -SCBT QC
Date published: 2015-01-17
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