Date published: 2025-10-15

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m-IgG2b BP-HRP: sc-542741

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Datasheets
  • mouse IgG2b binding protein (m-IgG2b BP) conjugated to Horseradish Peroxidase (HRP)
  • supplied at 50 µg in 0.5 ml volume
  • Highly recommended alternative to conventional anti-mouse IgG secondary antibodies for chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
  • Suitable for binding to most, but not all, mouse IgG2b immunoglobulins; not suitable for use with mouse monoclonal IgG1, IgG2a, IgG3, IgM, IgA or IgE antibodies
  • Not cross reactive with human, rat or goat IgG antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
  • Also see our new m-IgG2b BP-CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-mouse secondary antibody fluorescent dye conjugates.

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Mouse IgG2b binding protein (m-IgG2b BP) conjugated to horseradish peroxidase (HRP) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB) and immunohistochemical (IHC) signal enhancement. Mouse IgG2b binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgG2b binding protein (m-IgG2b BP) is suitable for for binding to most, but not all mouse monoclonal IgG2b antibodies; not suitable for use with mouse monoclonal IgG1, IgG2a, IgG3, IgM, IgA or IgE antibodies. Not cross reactive with human, rat or goat IgG antibodies.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgG2b BP-HRP

sc-542741
50 µg/0.5 ml
$92.00

I purchased MCT2 (sc-166925) monoclonal antibody and I wanted to run a western blot. What secondary is the best to run a western blot?

Asked by: Naomi
Thank you for your question. Here are the preferred secondary antibodies : https://www.scbt.com/p/m-igg2b-binding-protein-hrp https://www.scbt.com/p/m-igg-kappa-bp-hrp
Answered by: BlakeJ
Date published: 2024-07-29
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Rated 5 out of 5 by from Great for chemiluminescent western blots!This detection reagent worked well for detecting HMG-1 (D-3) antibody, sc-518191, in a chemiluminescent western blot assay. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Good antibodyThis secondary antibody allows the detection of nice, sharp bands in chemiluminescence Western blotting (WB [ECL]).
Date published: 2022-02-18
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m-IgG2b BP-HRP is rated 5.0 out of 5 by 2.
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