CD45 Antibody (T29/33) recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and FCM

CD45 Antibody (T29/33): sc-18901

CD45 Antibody (T29/33) is rated 4.5 out of 5 by 6.
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Datasheets

    • CD45 Antibody (T29/33) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
    • raised against the human cell line, CCRF-CEM
    • recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD45 (T29/33): sc-18901 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (T29/33): sc-18901 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Using feline mature red blood cells Using feline mature red blood cells, was not able to obtain successful results using FCM. Used FITC and PE conjugated antibodies.
Date published: 2015-06-26
Rated 5 out of 5 by from Excellent FCM data of BJAB cells Excellent FCM data of BJAB cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Bands at expected MW present in western blot Bands at expected MW present in western blot analysis of CD45 expression in Jurkat, CCRF-CEM and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Beautiful immunofluorescence membrane staining Beautiful immunofluorescence membrane staining in methanol-fixed Jurkat cells. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Successfully immunoprecipitated CD45 from Successfully immunoprecipitated CD45 from human B cells and verified via WB. -SCBT Publication Review
Date published: 2015-01-31
Rated 5 out of 5 by from Excellent FCM data of Jurkat cells Excellent FCM data of Jurkat cells. -SCBT QC
Date published: 2013-06-16
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