CD45 Antibody (OX1) recommended for detection of monomorphic determinant of CD45 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA

CD45 Antibody (OX1): sc-53045

CD45 Antibody (OX1) is rated 4.0 out of 5 by 2.
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Datasheets

    • CD45 Antibody (OX1) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
    • raised against full length thymocyte membrane glycoproteins of rat origin
    • recommended for detection of monomorphic determinant of CD45 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD45 (OX1): sc-53045 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (OX1): sc-53045 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from FACS publication in rat heart samples FACS publication in rat heart samples. -SCBT Publication Review
Date published: 2015-04-24
Rated 4 out of 5 by from Produced robust Indirect FCM data for rat Produced robust Indirect FCM data for rat peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2014-07-26
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