CD45 Antibody (30-F11) recommended for detection of CD45 of mouse origin by WB, IP, IF, IHC(P) and FCM

CD45 Antibody (30-F11): sc-53665

CD45 Antibody (30-F11) is rated 4.4 out of 5 by 8.
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Datasheets

    • CD45 Antibody (30-F11) is a rat monoclonal IgG2b, cited in 14 publications, provided at 200 µg/ml
    • raised against thymus and spleen of mouse origin
    • CD45 Antibody (30-F11) is recommended for detection of CD45 of mouse origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD45 Antibody (30-F11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (30-F11): sc-53665.

Do you have a product I can use as an isotype for the CD45 Antibody (30-F11) Alexa Fluor® 790 (a Rat IgG2b-AF790)? Thanks for your answer!

Asked by: Teresa_G
Thanks for your question. Unfortunately, at the moment we don´t offer a conjugated isotype from the requested species. Please contact technical service if you need any other information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-02-08
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Rated 5 out of 5 by from 抗体不错 我们实验室之前经常买这个公司的抗体,最近也购买了一些,打算申请一个CD45de 试用装,好的话再买它
Date published: 2019-09-21
Rated 5 out of 5 by from Excellent antibody I bought this about a month ago, I have used it for paraffin embedded samples of mice oral tissues and the leukocytes were very clear.
Date published: 2019-08-27
Rated 4 out of 5 by from 功能不错 试用了小样,也购买了正式装,做流式和免疫荧光是一般调到最高稀释浓度才能得到结果,虽然效价似乎不高,但是贵在能够用在多种实验和种属,保存也方便
Date published: 2018-02-06
Rated 4 out of 5 by from 功能较多样化 一个月前买的,可以用在多种实验中,种属适应范围刚好打到我们的实验要求,虽然实验中需要的浓度略高,但是效果还可以
Date published: 2018-02-06
Rated 5 out of 5 by from good wb result The antibody react with the cd45 in human leukocyte cell,it shows positive expression , I observed the specific band by dilution 1:1000.
Date published: 2017-07-08
Rated 5 out of 5 by from Good choice for FCM This rat monoclonal, CD45 Antibody (30-F11), performs well for detection of mouse CD45 in flow cytometry applications and is readily available conjugated to PE and FITC.
Date published: 2017-01-26
Rated 3 out of 5 by from Faint band observed at correct MW in western Faint band observed at correct MW in western blot analysis of CD45 expression in mouse PBL whole cell lysate. -SCBT QC
Date published: 2015-01-30
Rated 4 out of 5 by from Clear Indirect FCM data of mouse peripheral Clear Indirect FCM data of mouse peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2014-11-20
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