Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD45RC Double Nickase Plasmid (h): sc-400245-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD45 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD45 Double Nickase Plasmid (h) and CD45 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTPRC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD45 Antibody (35-Z6): sc-1178
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD45RC Double Nickase Plasmid (h) | sc-400245-NIC | 20 µg | $410.00 | |||
CD45 Double Nickase Plasmid (h2) | sc-400245-NIC-2 | 20 µg | $410.00 |
PTPRC encodes CD45RC, an isoform of the leukocyte common antigen (CD45) receptor-type protein tyrosine phosphatase that tunes signaling thresholds in hematopoietic cells. By dephosphorylating Src family kinases and other proximal components, CD45RC modulates antigen receptor signaling, cytokine responsiveness, and downstream pathways such as MAPK/ERK and JAK/STAT that shape lymphocyte activation and differentiation. Alternative splicing of PTPRC generates CD45 isoforms with distinct immunophenotypic distributions, making CD45RC useful for dissecting immune cell subsets and activation states. Dysregulated CD45 phosphatase activity and altered isoform expression have been linked to immune dysfunction and inflammatory disease mechanisms, supporting its relevance in immunology and hematologic research models.
CD45 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTPRC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTPRC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTPRC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTPRC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.