CD45 Antibody (I3/2.3) recommended for detection of CD45 of mouse origin by WB, IP, IF and FCM

CD45 Antibody (I3/2.3): sc-52491

CD45 Antibody (I3/2.3) is rated 4.3 out of 5 by 4.
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Datasheets

    • CD45 Antibody (I3/2.3) is a rat monoclonal IgG2b, cited in 6 publications, provided at 200 µg/ml
    • recommended for detection of CD45 of mouse origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD45 (I3/2.3): sc-52491 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (I3/2.3): sc-52491 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works as expected in WB Acceptable WB data for WBBYDP and TK-1 whole cell lysates at a 1: 200 dilution.
Date published: 2017-02-01
Rated 4 out of 5 by from Used this in IF and saw positive signal in Used this in IF and saw positive signal in mouse cells.
Date published: 2015-09-23
Rated 4 out of 5 by from Published using this antibody for immunofluorescence Published using this antibody for immunofluorescence microscopy. -SCBT Publication Review
Date published: 2015-01-14
Rated 4 out of 5 by from Produced wonderful Indirect FCM data for Produced wonderful Indirect FCM data for mouse peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2013-09-27
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