CD45 Antibody (F10-89-4) recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD45 Antibody (F10-89-4): sc-52490

CD45 Antibody (F10-89-4) is rated 3.8 out of 5 by 5.
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Datasheets

    • CD45 Antibody (F10-89-4) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
    • raised against T lymphocytes of human origin
    • recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD45 (F10-89-4): sc-52490 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (F10-89-4): sc-52490 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good Indirect FCM data of human peripheral Good Indirect FCM data of human peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2015-06-01
Rated 3 out of 5 by from Faint band present at expected MW in western Faint band present at expected MW in western blot analysis of CD45 expression in human PBL whole cell lysate. -SCBT QC
Date published: 2015-05-06
Rated 4 out of 5 by from Clean bands in WB with GA Clean bands in WB with GA-10 and Ramos whole cell lysates. -SCBT QC
Date published: 2015-02-22
Rated 4 out of 5 by from Published IF data in rat MSCs Published IF data in rat MSCs. -SCBT Publication Review
Date published: 2015-02-04
Rated 4 out of 5 by from Clear FCM data of Jurkat cells Clear FCM data of Jurkat cells. -SCBT QC
Date published: 2014-08-22
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