CD45RA Antibody (HI100): sc-19664

CD45RA Antibody (HI100) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize the CD45RA isoform of the CD45 protein. CD45RA is a transmembrane glycoprotein localized on the surface of hematopoietic cells, particularly naive T cells, where it plays a crucial role in regulating immune cell signaling and function. Positioned on the cell membrane, CD45RA facilitates the interaction between T-cell receptors (TCR) and their ligands, thereby modulating T-cell activation, proliferation, and differentiation. This precise localization is essential for distinguishing naive T cells from memory T cell populations, enabling researchers to study immune responses and the development of immunological memory effectively. The CD45 protein itself functions as a vital protein tyrosine phosphatase, responsible for dephosphorylating key tyrosine residues on Src family kinases such as Lck and Fyn. This enzymatic activity is fundamental for initiating and sustaining the downstream signaling cascades that drive T-cell responses, making CD45RA indispensable for effective immune system functioning. The CD45RA isoform arises from alternative splicing of the CD45 gene, resulting in unique extracellular domains that confer specific functional properties while maintaining a conserved intracellular phosphatase domain. This structural variation allows for specific interactions that are critical for the identification and characterization of naive T cells. CD45RA (HI100) monoclonal antibody is recommended for use in human samples and is compatible with a wide range of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). This versatility makes anti-CD45RA antibody (HI100) an essential tool for researchers aiming to explore immune cell functions, develop targeted therapies, and advance the understanding of immune system dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.