EGFR Antibody (3i4) recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF and IHC(P)

EGFR Antibody (3i4): sc-71032

EGFR Antibody (3i4) is rated 3.0 out of 5 by 2.
  • y_2020, m_11, d_30, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_71032, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 116ms
  • REVIEWS, PRODUCT
5
1
4
0
3
0
2
0
1
1

Datasheets

    • EGFR Antibody (3i4) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 985-996 of EGFR of human origin
    • recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See EGFR (A-10): sc-373746 for EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What blocking solution should I use with EGFR (3i4): sc-71032 monoclonal antibody for Western blot?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
  • y_2020, m_11, d_30, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_71032, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 115ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Bad antibody We received this sample antibody as a replacement for a previous antibody that has now been discontinued. We have validated our samples on multiple proteomic platforms to confirm the presence/absence of EGFR. Despite multiple attempts to optimize the antibody for Western Blot, our lab has not been successful validating our samples by Western Blot. I would not recommend this antibody for Western Blotting purposes.
Date published: 2018-08-27
Rated 5 out of 5 by from Good specificity and signal strength for Good specificity and signal strength for western blot analysis of EGFR expression in A-431 whole cell lysate. - SCBT QC
Date published: 2013-04-03
  • y_2020, m_11, d_30, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_71032, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.