p-EGFR Antibody (11C2) recommended for detection of Tyr 1045 phosphorylated EGFR of mouse, rat, human and canine origin by WB and IP

p-EGFR Antibody (11C2): sc-57541

p-EGFR Antibody (11C2) is rated 5.0 out of 5 by 1.
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Datasheets

    • p-EGFR Antibody (11C2) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 50 µg/0.5 ml
    • raised against an EGFR phosphopeptide of human origin
    • recommended for detection of Tyr 1045 phosphorylated EGFR of mouse, rat, human and canine origin by WB and IP
    • See EGFR (A-10): sc-373746 for p-EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using p-EGFR (11C2): sc-57541 monoclonal antibody for immunoprecipitation, how much antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-04-05
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Rated 5 out of 5 by from Great antibody Great antibody, single and strong band for western blot analysis of EGFR phosphorylation in serum starved MDA-MB-468 cells and serum starved MDA-MB-468 cells treated with 10 ng/ml EGF for 5 min, 15 min, 30 min, 1 hr, 2 hrs, 4 hrs and 8 hrs. - SCBT QC
Date published: 2014-10-01
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