Anti-EGFR Antibody (A-10) recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-EGFR Antibody (A-10): sc-373746

Anti-EGFR Antibody (A-10) is rated 4.6 out of 5 by 57.
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Datasheets

    • Anti-EGFR Antibody (A-10) is a mouse monoclonal IgG2a κ EGFR antibody, cited in 125 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin
    • Anti-EGFR Antibody (A-10) is recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-EGFR Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EGFR (A-10): sc-373746.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EGFR Antibody (A-10) for WB and IHC(P) applications. These reagents are now offered in bundles with EGFR Antibody (A-10) (see ordering information below).
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Does this ab recognize the mouse EGFR in tissus (IF), what is the dilution recommended? Thanks

Asked by: Any21
Thank you for your question. Yes, this antibody is recommended for IF/IHC on mouse tissue samples. We recommend a starting dilution of 1:50 for this antibody.
Answered by: Tech Service
Date published: 2019-09-27

Does this antibody against mutant forms of EGFR in mouse or human?

Asked by: Po Lee
Thank you for your question. EGFR Antibody (A-10): sc-373746 is specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin. EGFR proteins possessing this amino acid sequence should be recognized by this antibody without issue.
Answered by: Tech Support Europe
Date published: 2020-05-20

if I probe western blot with EGFR sc-373746, I don't have to against the blot with 2nd antibody. Is that right? Is this a phospho-EGFR antibody?

Asked by: Daisy II
This antibody is available unconjugated (sc-373476), in which case you must use a secondary anti-mouse IgG for detection. It is also available directly conjugated to HRP, and fluorescent probes, (sc-373476 HRP, etc.), in which case there is no secondary antibody needed for detection. This is not a phospho-specific EGFR antibody. It is reactive with total EGFR.
Answered by: Technical Support
Date published: 2020-05-20

HI, I need to do negative depletion of cells using an anti-EGF-receptor monoclonal antibody, mouse-human. can I use EGFR Antibody (A-10): sc-373746 for this purpose?

Asked by: Maria Vernon
Thank you for your question. We have not tested this antibody for any biological activity such as depletion, and so this is not a recommended application at this time. We do offer an alternative antibody which has been validated for use in biological studies, including neutralization, EGFR Antibody (R-1): sc-101, also provided azide-free for biological studies, sc-101 L.
Answered by: Technical Support
Date published: 2019-03-03

Is it positive for MCF-7 or MDA-MB-231 ?

Asked by: Gracess
Thank you for your question. We have published Western blot data on our website using this antibody in MDA-MB-231 whole cell lysates. We do not have any data in MCF-7.
Answered by: Technical Support
Date published: 2020-05-20

We recently ran the EGFR Antibody (A-10): sc-373746 and did not detect any banding on U87 lysates. What positive control do you recommend. We rant the lysate on a 4-12% MOPS PAGE....

Asked by: DrxFif
Thank you for your inquiry. We recommend serum starved Hela or A549 cells, or HepG2 cells. 4-12% gel should be fine. Please contact our Technical Service Department by phone, e-mail or live chat, and we'd be happy to help you troubleshoot the antibody.
Answered by: Tech Service
Date published: 2017-08-14

What is the recommended antigen retrieval method to be used with this antibody?

Asked by: mony8
Thank you for your question. We recommend using a sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this mouse monoclonal antibody, EGFR (A-10): sc-373746. A link to our recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-06-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-23
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Rated 4 out of 5 by from molto buono sono molto soddisfatto del prodotto ordinato come sample
Date published: 2021-02-22
Rated 5 out of 5 by from very good antibody very clean antibody for western blot. great antibody
Date published: 2020-12-01
Rated 1 out of 5 by from EGFR assay We failed to detect EGFR bands from human iPSC-derived DA neurons.
Date published: 2020-08-17
Rated 4 out of 5 by from Sensitive in WB detection Detects efficiently EGFR in HeLa cells in WB (25 ug protein in a dilution 1:100).
Date published: 2020-06-12
Rated 4 out of 5 by from 想买 试用装需要随购买的试剂一起赠送。想买来做肿瘤药物的靶向制剂。不知能不能用来作为靶向制剂,请告知。十分感谢
Date published: 2020-05-20
Rated 4 out of 5 by from きれいに染まります HCS2細胞株の免疫染色に用いました。1:100~1:500で振ってそめて、いずれもきれいにそまります。
Date published: 2020-03-30
Rated 5 out of 5 by from Very good antibody I have been using this antibody for a year now and it works perfectly well in detecting total EGFR in U87 cell-line at 1:500 dilution in 5% non-fat dry milk/TBST. I will recommed this antibody
Date published: 2019-11-21
Rated 5 out of 5 by from Good to use This antibody is high specific and high sensitivity in human cell lines. Highly recommend this antibody
Date published: 2019-10-23
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