EGFR Antibody (528) recommended for detection of EGFR of mouse, rat and human origin by WB (non-reducing), IP, IF and FCM

EGFR Antibody (528): sc-120

EGFR Antibody (528) is rated 4.4 out of 5 by 17.
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Datasheets

    • EGFR Antibody (528) is a mouse monoclonal IgG2a κ, cited in 315 publications, provided at 200 µg/ml
    • mapping to a cell surface epitope of EGFR of human origin
    • Anti-EGFR Antibody (528) is recommended for detection of EGFR of mouse, rat and human origin by WB (non-reducing), IP, IF and FCM; also reactive with additional species, including canine
    • EGFR Antibody (528) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EGFR (528): sc-120.

Hi, The EGFR Antibody (528) cat. # sc-120 is mentioned to be used for WB under non-reducing conditions. Can this antibody detect EGFR in their reduced forms? Thanks

Asked by: Anonymous
Thank you for your question. For western blot application the (528) clone is likely to be di-sulfide bridge dependent therefore we recommend running the western blot under non-reducing conditions. If you have any further questions, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2020-11-12

potrei ricevere un FREE 10 µg sample di EGFR (528): sc-120? Vorrei testarlo su alcune linee cellulari.

Asked by: Rosanna16
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-02-06

Can this antibody be used for immunohistochemical staining of mouse tissue?

Asked by: AbPolly
Thank you for your inquiry. This antibody can be used to stain mouse tissue, however we recommend using one of the directly conjugated forms. This EGFR antibody is available conjugated to PE, FITC, AF488, AF647 and more.
Answered by: Technical Service
Date published: 2016-12-22

What secondary is recommended for this primary when using a fluorescent imaging system in Western Blot?

Asked by: AbPolly
Thank you for your question. We recommend using one of our two new Near-Infrared CruzFluor mouse IgG binding proteins, m-IgGκ BP-CFL 680 sc-516180 or m-IgGκ BP-CFL 790 sc-516181. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-22
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Rated 1 out of 5 by from Antibodies not specific The N-terminal antibody doesn't give specific band.
Date published: 2020-10-21
Rated 4 out of 5 by from きれいに染まります。 HCS2の免疫染色に使用しました。1:100~1:1500の希釈でいずれもきれいに染まります。バックグラウンドも問題ないです。
Date published: 2020-03-30
Rated 5 out of 5 by from Works for flow cytometry EGFR antibody works for flow cytometry on HFF cells
Date published: 2018-11-01
Rated 4 out of 5 by from Works well Gave large bands when used at 1:200 in mouse lysates.
Date published: 2018-03-23
Rated 1 out of 5 by from Terrible, I do not trust this antibody I ran two identical western blots side by side with 100 ug of protein from tissue. I compared this antibody to EGFR (A-10) sc-373746 and the banding pattern was completely different. If I chose to analyze my data with this antibody it would have completely biased my data and results.
Date published: 2018-02-21
Rated 5 out of 5 by from Good for immunostaining I brought this antibody one month ago and tried it for immunofluorescent staining. It works well and is very specific. Happy with this product
Date published: 2017-10-31
Rated 5 out of 5 by from Suitable for IHC detection Good IHC staining in human pancreas tissue, the result is very satisfactory.
Date published: 2017-05-28
Rated 5 out of 5 by from effect is obvious Worked great for Western Blot, use 1:500 dilution.
Date published: 2017-05-10
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