EGFR Antibody (F-9) recommended for detection of EGFR of human origin by WB, IP, IF, IHC(P) and ELISA

EGFR Antibody (F-9): sc-377073

EGFR Antibody (F-9) is rated 4.5 out of 5 by 4.
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Datasheets

    • EGFR Antibody (F-9) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1131-1169 within a C-terminal cytoplasmic domain of EGFR of human origin
    • recommended for detection of EGFR of human origin by WB, IP, IF, IHC(P) and ELISA
    • See EGFR (A-10): sc-373746 for EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What application is the blocking peptide sc-377073 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24

Hi there, I'm Dr. Chang from UBIA, Taiwan, and seeking anti-human EGFR Ab, but no cross-reactivity to mouse EGFR. And I will apply in WB and IHC-p experiment.If you have any suitable product fits my request, please don't hesitate to contact with me. Thank

Asked by: Morris
Thank you for your question. Yes, we would recommend using our mouse monoclonal, EGFR Antibody (F-9): sc-377073. This antibody has been tested by Western blot in samples of mouse and human origin, and only shows reactivity with human EGFR.
Answered by: Technical Support
Date published: 2017-01-06
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Rated 5 out of 5 by from Worked well in WB at 1:200 dilution Worked well in WB at 1:200 dilution.
Date published: 2015-06-24
Rated 5 out of 5 by from Good signal strength in HeLa and BT Good signal strength in HeLa and BT-20 whole cell lysates. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, membrane staining in MeOH fixed HeLa cells. -SCBT QC
Date published: 2015-01-05
Rated 3 out of 5 by from Detected signal at correct molecular weight Detected signal at correct molecular weight with some background in EGF treated A-431 whole cell lysate. -SCBT QC
Date published: 2013-08-07
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