EGFR Antibody (C-2) recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

EGFR Antibody (C-2): sc-377229

EGFR Antibody (C-2) is rated 4.7 out of 5 by 7.
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Datasheets

    • EGFR Antibody (C-2) is a mouse monoclonal IgG2a κ, cited in 9 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1131-1169 within a C-terminal cytoplasmic domain of EGFR of human origin
    • Anti-EGFR Antibody (C-2) is recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-EGFR Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EGFR (C-2): sc-377229.

What is the predicted size of this protein for western blot?

Asked by: JDM4
Thank you for your question. The molecular weight is predicted to be 170 kDa.
Answered by: Technical Support
Date published: 2019-01-06

I need to use an antibody to detect the protein in samples of non-mammalian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11

In mouse tissue (Mus musculus) what would be an appropriate positive control to use?

Asked by: RTMac
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Rated 4 out of 5 by from Works fantastic! This antibody is very good for IF. 1:50 is a very good concentration to use
Date published: 2019-09-25
Rated 5 out of 5 by from Nice strong band! Yielded a strong band when used at 1:200 probing mouse and human lysates via WB.
Date published: 2018-03-23
Rated 5 out of 5 by from Superb WB and IHC Great product for western blot applications; also outstanding IHC
Date published: 2017-01-29
Rated 5 out of 5 by from Great antibody! The EGFR Antibody (C-2): sc-377229 performs very well for IHC applications when using formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-01-13
Rated 5 out of 5 by from Good EGFR control antibody for CRISPR/Cas9 Good EGFR control antibody for CRISPR/Cas9 KO experiment -SCBT QC
Date published: 2015-04-10
Rated 4 out of 5 by from Blowout band in EGF treated A Blowout band in EGF treated A-431 and good signal with BT-20 whole cell lysates. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Great antibody for paraffin embedded sections Great antibody for paraffin embedded sections. The sc-377229, EGFR antibody gives a strong membrane and cytoplasmic signal in human vagina tissue. -SCBT QC
Date published: 2014-05-11
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