EGFR Antibody (R-1): sc-101

EGFR Antibody (R-1) is a mouse monoclonal IgG2b kappa light chain antibody raised against human epidermal growth factor receptor (EGFR), a crucial receptor tyrosine kinase involved in cell signaling and growth regulation. EGFR mouse monoclonal antibody (R-1) recognizes an epitope within the EGFR immunogen sequence and demonstrates broad species cross-reactivity, detecting EGFR in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM) applications. Anti-EGFR monoclonal antibody (R-1) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. EGFR functions as a sophisticated signal transducer on the cell surface, where EGFR responds to specific ligands including epidermal growth factor (EGF) and transforming growth factor alpha (TGFα). Upon ligand binding, EGFR undergoes dimerization and activates intrinsic tyrosine kinase activity, triggering downstream signaling cascades such as RAS-RAF-MAPK and PI3K-AKT pathways. These pathways regulate fundamental cellular processes including proliferation, survival, differentiation, and play essential roles in organ morphogenesis and tissue repair. When EGFR signaling becomes dysregulated, particularly through overexpression or mutation, the resulting aberrant activation can promote tumorigenesis by driving uncontrolled cell growth, invasion, and metastasis. Through EGFR′s prevalent expression in many carcinomas, EGFR serves as an important therapeutic target, as inhibiting EGFR function can induce cancer cell cycle arrest and apoptosis. EGFR (R-1) antibody provides researchers with a valuable tool for investigating these complex signaling mechanisms and developing targeted cancer therapies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EGFR Antibody (R-1) has 114 citations in a variety of scientific publications.

Primary antibodies like Anti-EGFR Antibody (R-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EGFR Antibody (R-1) Specifications

Alternate Product Name: EGFR

Product Clone Name: R-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 6-273

Epitope Target: EGFR

Epitope Species: human

Product Reactivity/Detection: EGFR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 114 citations in various scientific publications

Available Conjugates/Forms for EGFR Antibody (R-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin

The preferred EGFR antibody is EGFR Antibody (528): sc-120.