EGFR Antibody (R-1) recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF and FCM

EGFR Antibody (R-1): sc-101

EGFR Antibody (R-1) is rated 4.9 out of 5 by 10.
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Datasheets

    • EGFR Antibody (R-1) is a mouse monoclonal IgG2b κ, cited in 87 publications, provided at 200 µg/ml
    • raised against human epidermoid carcinoma cell line A431, with epitope mapping between amino acids 6-273 of human EGFR
    • Anti-EGFR Antibody (R-1) is recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF and FCM
    • Anti-EGFR Antibody (R-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • also provided azide-free for biological studies, sc-101 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EGFR (R-1): sc-101.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for EGFR Antibody (R-1) for WB applications. This reagent is now offered in a bundle with EGFR Antibody (R-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can I get a free sample of an antibody (sc-365829) to try for my application?  Can I try several of your EGF-R antibodies to determine the optimal reagent for my use (sc-120 & sc-101)? Thank You

Asked by: MAntczak
We currently offer sample sizes for all 3 of these EGFR antibodies. Please contact our Technical Service Department for current information on how to receive a free 10 ug monoclonal antibody sample.
Answered by: Technical Support
Date published: 2020-04-09

bonjour,  savez-vous si la reconnaissance de la protéine par l'anticorps est dépendante des N-glycosylations de l'EGFR ?

Asked by: Serge
merci pour votre question. Veuillez contacter notre Service Technique par email [email protected] pour plus d'informations sur ce produit.
Answered by: Tech Support Europe
Date published: 2019-12-16

How can EGFR Antibody (R-1): sc-101 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. EGFR Antibody (R-1): sc-101 is currently available conjugated to phycoerythrin (sc-101 PE), fluoroscein (sc-101 FITC), Alexa Fluor® 488 (sc-101 AF488), Alexa Fluor® 647 (sc-101 AF647), PerCP (sc-101 PerCP) or Alexa Fluor® 405 (sc-101 AF405)
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Great features I bought this a month ago and I'm so happy that I did
Date published: 2020-11-19
Rated 4 out of 5 by from Worked well Gave nice strong bands for WB at 1:200. Used with mouse samples.
Date published: 2018-03-22
Rated 5 out of 5 by from A good choice for EGFR detection This antibody is a great choice. The IF data is features strong and specific signals while Western blots will result in bright clean bands. Researchers have published with this antibody over 30 times.
Date published: 2017-01-17
Rated 5 out of 5 by from Outstanding!! Great IF and western blot results;far superior to polyclonal alternatives
Date published: 2017-01-06
Rated 5 out of 5 by from no comment no comment
Date published: 2015-05-15
Rated 5 out of 5 by from Worked well for Western blot with HUVEC samples Worked well for Western blot with HUVEC samples. -SCBT Publication Review
Date published: 2015-03-27
Rated 5 out of 5 by from IHC publication with human esophagus adenocarcinoma. -SCBT Publication Review
Date published: 2015-02-13
Rated 5 out of 5 by from Great antibody for mouse IP Great antibody for mouse IP, published using MCF-7. -SCBT Publication Review
Date published: 2015-01-29
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