p-EGFR Antibody (1H9) recommended for detection of Ser 1047 phosphorylated EGFR of mouse, rat, human and canine origin by WB, IP and IF

p-EGFR Antibody (1H9): sc-57543

p-EGFR Antibody (1H9) is rated 4.0 out of 5 by 3.
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Datasheets

    • p-EGFR Antibody (1H9) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against synthetic phosphopeptide corresponding to amino acid residues surrounding serine 1047 of EGFR of human origin
    • recommended for detection of Ser 1047 phosphorylated EGFR of mouse, rat, human and canine origin by WB, IP and IF
    • See EGFR (A-10): sc-373746 for p-EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What is the recommended starting dilution for Western blot for p-EGFR (1H9): sc-57543 monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
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Rated 3 out of 5 by from Weak band with some background for Western Weak band with some background for Western blot analysis using untreated and lambda protein phosphatase treated SK-OV-3 whole cell lysates. - SCBT QC
Date published: 2015-01-22
Rated 4 out of 5 by from Worked well with serum starved PMA Worked well with serum starved PMA-treated, Sorbit-treated, and Anisomycin-treated Hep G2 whole cell lysates. - SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Good specificity and signal strength for WB in A Good specificity and signal strength for WB in A-431 whole cell lysate. - SCBT QC
Date published: 2014-10-03
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