Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EGFR Double Nickase Plasmid (m): sc-420131-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EGFR Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EGFR Double Nickase Plasmid (m) and EGFR Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Egfr. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EGFR Antibody (A-10): sc-373746
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EGFR Double Nickase Plasmid (m) | sc-420131-NIC | 20 µg | $410.00 | |||
EGFR Double Nickase Plasmid (m2) | sc-420131-NIC-2 | 20 µg | $410.00 |
Mouse EGFR (Egfr) is a receptor tyrosine kinase that binds epidermal growth factor family ligands to trigger receptor dimerization, autophosphorylation, and downstream signaling. Activated EGFR engages MAPK/ERK, PI3K–AKT, JAK/STAT, and PLCγ pathways to regulate proliferation, survival, differentiation, and epithelial tissue homeostasis. In mouse models, Egfr-dependent signaling shapes developmental programs and wound responses, and altered EGFR activity is frequently used to study dysregulated growth factor signaling in oncology and inflammatory biology. EGFR also intersects with endocytic trafficking and feedback regulators that tune signaling amplitude and duration, supporting investigations of receptor turnover and signal transduction dynamics.
EGFR Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Egfr locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Egfr. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Egfr function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Egfr-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.