p-EGFR Antibody (F-3) recommended for detection of Tyr 1092 phosphorylated EGFR of mouse and human origin and Tyr 1091 phosphorylated EGFR of rat origin (also designated as Tyr 1068) by WB, IP, IF and ELISA

p-EGFR Antibody (F-3): sc-377547

p-EGFR Antibody (F-3) is rated 3.5 out of 5 by 12.
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Datasheets

    • p-EGFR Antibody (F-3) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids Tyr 1068 Tyr 1068 of EGFR of human origin
    • p-EGFR Antibody (F-3) is recommended for detection of Tyr 1092 phosphorylated EGFR of mouse and human origin and Tyr 1091 phosphorylated EGFR of rat origin (also designated as Tyr 1068) by WB, IP, IF and ELISA; also reactive with additional species, including canine
    • Anti-p-EGFR Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-EGFR (F-3): sc-377547.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for p-EGFR Antibody (F-3) for WB applications. This reagent is now offered in a bundle with p-EGFR Antibody (F-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Is this antibody specific for the phosphorylated protein, and will not cross-react with the non-phosphorylated form of the protein?

Asked by: TinTin
Yes, p-EGFR (F-3): sc-37754 is specific for p-EGFR phosphorylated at Tyrosine 1092. The antibody has been validated through a series Western blot experiments using lambda phosphatase treated lysates to demonstrate phospho-specificity.
Answered by: Tech Service
Date published: 2017-01-27
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Rated 2 out of 5 by from Not good This one did not wrok well as the previous one we purchased from Santa, even this is a mono-ab
Date published: 2018-03-30
Rated 4 out of 5 by from Great results great results for WB. Recommend using 1:200 dilution and exposing for longer period to get nice dark bands
Date published: 2018-03-22
Rated 1 out of 5 by from Not good for WB Didn't received good band with this antibody in WB.
Date published: 2018-02-14
Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 2 out of 5 by from OK It did not work well as our old ab purchased from Santa cruz! Sadly the old one discountinued!
Date published: 2017-10-03
Rated 5 out of 5 by from work perfect The antibody was great, its signal was were strong, and the backgroud was very clean.
Date published: 2017-09-22
Rated 5 out of 5 by from clear bands achieved I have performed western blotting on rat aorta primary endothelial cells and achieved clear bands
Date published: 2017-09-14
Rated 3 out of 5 by from The backgroud is not quite clear. 1:100 diluted for wb of rat protein,the band is not quite strong,and the backgroud is not quite clear.
Date published: 2017-08-28
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