p-EGFR Antibody (9H2) recommended for detection of Tyr 1173 phosphorylated EGFR of mouse, rat, human and canine origin by WB, IP and IF

p-EGFR Antibody (9H2): sc-57545

p-EGFR Antibody (9H2) is rated 4.7 out of 5 by 7.
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Datasheets

    • p-EGFR Antibody (9H2) is a mouse monoclonal IgG1, cited in 6 publications, provided at 50 µg/0.5 ml
    • raised against an EFGR phosphopeptide of human origin
    • recommended for detection of Tyr 1173 phosphorylated EGFR of mouse, rat, human and canine origin by WB, IP and IF; non cross-reactive with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins
    • See EGFR (A-10): sc-373746 for p-EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-EGFR (9H2): sc-57545.

How much protein do you recommend loading on the gel for Western blot detection using p-EGFR (9H2): sc-57545 monoclonal antibody?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 4 out of 5 by from Good The antibody was used for Western Blot in breast carcinoma cells, it works at a concentration of 1: 100.
Date published: 2020-10-16
Rated 4 out of 5 by from Works well used for WB looking at mouse tissue samples using 1:200. Provides a faint but clear band.
Date published: 2018-03-22
Rated 5 out of 5 by from Great antibody i got this antibody as free trial size and worked so well on mouse mammary tissue lysates.
Date published: 2017-12-15
Rated 5 out of 5 by from Great phospho-detection Great, published antibody for p-EGFR detection recommended for use in mouse, rat, human, and canine. Strong bands in WB for phosphorylated samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Working great with dSTORM IF with HeLa and Working great with dSTORM IF with HeLa and Cos7 cells.
Date published: 2015-03-30
Rated 5 out of 5 by from Good Western blot analysis of EGFR phosphorylation Good Western blot analysis of EGFR phosphorylation in non-stimulated, EGF stimulated and pervanadate stimulated A549 whole cell lysates. - SCBT QC
Date published: 2014-02-09
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