p-EGFR Antibody (12A3) recommended for detection of Tyr 845 phosphorylated EGFR of mouse, rat and human origin by WB and IP

p-EGFR Antibody (12A3): sc-57542

p-EGFR Antibody (12A3) is rated 4.5 out of 5 by 4.
  • y_2020, m_11, d_29, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_57542, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 131ms
  • REVIEWS, PRODUCT
5
2
4
2
3
0
2
0
1
0

Datasheets

    • p-EGFR Antibody (12A3) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against an EGFR phosphopeptide of human origin
    • recommended for detection of Tyr 845 phosphorylated EGFR of mouse, rat and human origin by WB and IP; non cross-reactive with the non-phosphorylated EGFR nor with unrelated tyrosine-phosphorylated proteins
    • See EGFR (A-10): sc-373746 for p-EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-EGFR (12A3): sc-57542.

At what dilution should the antibody be used for a western blot (in 5% non-fat dry milk/TBST) ?

Asked by: MmeSprits
Thank you for your question. The recommended starting dilution for this antibody is 1:100 for Western blot applications.
Answered by: Tech Service
Date published: 2020-02-28

Is p-EGFR (12A3): sc-57542 monoclonal antibody specific for the phosphorylated protein, and will not cross-react with the non-phosphorylated form of the protein?

Asked by: jerojero
Yes, p-EGFR (12A3): sc-57542 is specific for p-EGFR phosphorylated at Tyrosine 845. The antibody has been validated through a series Western blot experiments using stimulated, non-stimulated and pervanadate treated lysates to demonstrate phospho-specificity.
Answered by: Technical Support
Date published: 2017-04-05
  • y_2020, m_11, d_30, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_57542, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 87ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works great! Highly recommend. used at 1:200 for human and mouse samples and worked great but I recommend longer exposure times.
Date published: 2018-03-22
Rated 5 out of 5 by from Great for Tyr 845 phosphorylated EGFR This monoclonal, p-EGFR (12A3): sc-57542, is specific for Tyr 845 phosphorylated EGFR and we induced phosphorylation using both EGF-stimulated, and pervanadate treated A549 and Hep G2 whole cell lysates.
Date published: 2017-02-01
Rated 4 out of 5 by from Works well with a little background using EGF Works well with a little background using EGF-stimulated and pervanadate treated A549 whole cell lysates by WB. - SCBT QC
Date published: 2015-02-09
Rated 4 out of 5 by from Good signal strength with a little background Good signal strength with a little background for western blot analysis of EGFR phosphorylation in EGF stimulated and pervanadate treated Hep G2 whole cell lysates.- SCBT QC
Date published: 2014-10-21
  • y_2020, m_11, d_29, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_57542, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.