EGFR Antibody (3H2094) recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF and IHC(P)

EGFR Antibody (3H2094): sc-71033

EGFR Antibody (3H2094) is rated 5.0 out of 5 by 3.
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Datasheets

    • EGFR Antibody (3H2094) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
    • raised against amino acids 985-996 of EGFR of human origin
    • recommended for detection of EGFR of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See EGFR (A-10): sc-373746 for EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What blocking solution should I use with EGFR (3H2094): sc-71033 monoclonal antibody for Western blot?

Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 5 out of 5 by from Specific and Sensitive Specifity to EGFR confirmed by cell lysate treatment with 17-AAG resulting in downregulation of EGFR expression - great choice.
Date published: 2017-02-02
Rated 5 out of 5 by from Good WB data with a little background in Good WB data with a little background in untreated and 17-AAG treated T98G whole cell lysates. - SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Works well on A Works well on A-431 whole cell lysate by western blot. - SCBT QC
Date published: 2013-04-09
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