p-EGFR Antibody (8B8) recommended for detection of Tyr 1086 phosphorylated EGFR of human origin by WB and IP

p-EGFR Antibody (8B8): sc-81490

p-EGFR Antibody (8B8) is rated 4.0 out of 5 by 1.
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Datasheets

    • p-EGFR Antibody (8B8) is a mouse monoclonal IgG2b provided at 50 µg/0.5 ml
    • raised against synthetic phosphopeptide corresponding to amino acid residues surrounding tyrosine 1086 of EGFR of human origin
    • recommended for detection of Tyr 1086 phosphorylated EGFR of human origin by WB and IP; non cross-reactive with non-phosphorylated EGFR or unrelated p-Tyr proteins
    • See EGFR (A-10): sc-373746 for p-EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What is the recommended starting dilution for Western blot for p-EGFR (8B8): sc-81490 monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
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Rated 4 out of 5 by from Worked well in WB with NIH/3T3 and U87MG cells Worked well in WB with NIH/3T3 and U87MG cells -SCBT Publication Review
Date published: 2014-06-10
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