Anti-p-EGFR Antibody (10G12) recommended for detection of Tyrosine 1148 phosphorylated EGFR of human origin by WB and IP

Anti-p-EGFR Antibody (10G12): sc-81487

Anti-p-EGFR Antibody (10G12) is rated 4.0 out of 5 by 1.
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Datasheets

    • p-EGFR Antibody (10G12) is a mouse monoclonal IgG3, cited in 2 publications, provided at 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide surrounding tyrosine 1148 of EGFR of human origin
    • recommended for detection of Tyrosine 1148 phosphorylated EGFR of human origin by WB and IP
    • See EGFR (A-10): sc-373746 for p-EGFR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-EGFR Antibody (10G12). This work is in progress.
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Is p-EGFR (10G12): sc-81487 monoclonal antibody specific for the phosphorylated protein, and will not cross-react with the non-phosphorylated form of the protein?

Asked by: jenniferc
Yes, p-EGFR (10G12): sc-81487 is specific for p-EGFR phosphorylated at Tyrosine 1148. The antibody has been validated through a series Western blot experiments using EGF stimulated lysates to demonstrate phospho-specificity.
Answered by: Technical Support
Date published: 2017-04-05
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Rated 4 out of 5 by from Clean bands with no background with EGF treated Clean bands with no background with EGF treated whole cell lysates -SCBT QC
Date published: 2013-01-04
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