RNF213 Activators
RNF213 activators are a series of chemical compounds that amplify the functional ubiquitin ligase activity of RNF213 through the modulation of diverse cellular signaling pathways. Forskolin, which increases cAMP levels, indirectly augments the activity of RNF213 by activating PKA, leading to phosphorylation events that can enhance the ligase's function. This enhancement is mirrored by the action of MG132 and Epoxomicin, proteasome inhibitors that cause an accumulation of ubiquitinated proteins, thereby necessitating increased RNF213 ligase activity to maintain protein homeostasis. PMA, as a PKC activator, and Ionomycin, through its calcium ionophore activity, both lead to alterations in phosphorylation patterns, potentially enhancing RNF213's ubiquitin ligase activity. Additionally, the provision of zinc ions by ZnCl2 could act as a direct cofactor for RNF213, thus amplifying its enzymatic activity.
The second set of RNF213 activators includes LY294002, U0126, SB203580, and Okadaic Acid, each of which alters cellular signaling in a way that can indirectly enhance RNF213's activity. LY294002 suppresses PI3K, leading to compensatory ubiquitination activity, which RNF213 could be a part of, while U0126 and SB203580, as inhibitors of MEK and p38 MAPK respectively, may shift phosphorylation states that favor RNF213 activity. Okadaic Acid, inhibiting protein phosphatases, results in hyperphosphorylation, which could indirectly increase RNF213's ubiquitin ligase activity.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin raises intracellular cAMP, which activates PKA. PKA can then phosphorylate RNF213 or its substrates, which would enhance the E3 ubiquitin ligase activity of RNF213. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG132 is a proteasome inhibitor that leads to the accumulation of ubiquitinated proteins, thereby increasing the functional requirement for RNF213's ubiquitin ligase activity. | ||||||
Epoxomicin | 134381-21-8 | sc-201298C sc-201298 sc-201298A sc-201298B | 50 µg 100 µg 250 µg 500 µg | $134.00 $215.00 $440.00 $496.00 | 19 | |
Similar to MG-132, Epoxomicin inhibits the proteasome, which could lead to an increased need for RNF213's ubiquitin ligase activity to tag proteins for degradation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA is a PKC activator, which may phosphorylate RNF213 or its associated proteins, leading to an enhancement of RNF213's E3 ligase function. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin elevates intracellular calcium, which activates calcium-dependent enzymes that may modulate RNF213 activity through phosphorylation. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $47.00 | ||
ZnCl2 provides zinc ions that can act as a cofactor for RNF213, potentially enhancing its ubiquitin ligase activity. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 inhibits PI3K, potentially leading to enhanced RNF213 activity as the cell compensates by upregulating ubiquitination processes. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $88.00 $342.00 | 284 | |
SB203580 inhibits p38 MAPK, which could indirectly enhance RNF213 activity by affecting the phosphorylation of proteins within the RNF213 signaling pathway. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid inhibits PP1 and PP2A, which may result in the enhancement of RNF213 activity due to increased phosphorylation of its substrates. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin disrupts calcium homeostasis, which may enhance RNF213 activity via calcium-dependent signaling pathways influencing phosphorylation. | ||||||