Date published: 2025-11-22

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SB 203580 (CAS 152121-47-6)

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Alternate Names:
SB 203580 is also known as RWJ 64809.
Application:
SB 203580 is a pyridinyl imidazole and specific inhibitor that suppresses p38 mediated activation of MK2.
CAS Number:
152121-47-6
Purity:
≥98%
Molecular Weight:
377.43
Molecular Formula:
C21H16FN3OS
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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SB 203580 is a pyridinyl imidazole which suppresses the p38 mediated activation of MAPKAP kinase-2 (MAPKAP K2 or MK2), yet does not disrupt JNK activity. Mitogen-activated protein kinases (MAPK) are a group of serine and threonine-specific kinases sensitive to cellular stress, heat shock and proinflammatory cytokines. p38 MAPK is critical for normal immune and inflammatory responses as it regulates the expression of many cytokines, transcription factors, and cell surface receptors. SB 203580 inhibits the phosphorylation of heat shock protein 27 (HSP 27). SB 203580 may act to induce the activation of Raf-1. SB 203580 has also been shown to prevent the activation of PKB/Akt by inhibiting phosphoinositide-dependent protein kinase 1 (PDK1). SB 203580 is a useful tool to assess the physiological roles of p38, and for deriving and maintaining embryonic stem cells.


SB 203580 (CAS 152121-47-6) References

  1. Raf-1 is activated by the p38 mitogen-activated protein kinase inhibitor, SB203580.  |  Kalmes, A., et al. 1999. FEBS Lett. 444: 71-4. PMID: 10037150
  2. Effect of SB 203580 on the activity of c-Raf in vitro and in vivo.  |  Hall-Jackson, CA., et al. 1999. Oncogene. 18: 2047-54. PMID: 10321729
  3. Thioredoxin negatively regulates p38 MAP kinase activation and IL-6 production by tumor necrosis factor-alpha.  |  Hashimoto, S., et al. 1999. Biochem Biophys Res Commun. 258: 443-7. PMID: 10329406
  4. Pyridinylimidazole compound SB 203580 inhibits the activity but not the activation of p38 mitogen-activated protein kinase.  |  Kumar, S., et al. 1999. Biochem Biophys Res Commun. 263: 825-31. PMID: 10512765
  5. SB 203580, an inhibitor of p38 MAPK, abolishes infarct-limiting effect of ischemic preconditioning in isolated rabbit hearts.  |  Nakano, A., et al. 2000. Basic Res Cardiol. 95: 466-71. PMID: 11192367
  6. Preclinical pharmacokinetics of SB-203580, a potent inhibitor of p38 mitogen-activated protein kinase.  |  Ward, KW., et al. 2001. Xenobiotica. 31: 783-97. PMID: 11765141
  7. BMP4 supports self-renewal of embryonic stem cells by inhibiting mitogen-activated protein kinase pathways.  |  Qi, X., et al. 2004. Proc Natl Acad Sci U S A. 101: 6027-32. PMID: 15075392
  8. IL-1beta signaling in cat lower esophageal sphincter circular muscle.  |  Cao, W., et al. 2006. Am J Physiol Gastrointest Liver Physiol. 291: G672-80. PMID: 16645161
  9. SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1.  |  Cuenda, A., et al. 1995. FEBS Lett. 364: 229-33. PMID: 7750577
  10. Role for p38 mitogen-activated protein kinase in platelet aggregation caused by collagen or a thromboxane analogue.  |  Saklatvala, J., et al. 1996. J Biol Chem. 271: 6586-9. PMID: 8636072
  11. Pyridinyl imidazole inhibitors of p38 mitogen-activated protein kinase bind in the ATP site.  |  Young, PR., et al. 1997. J Biol Chem. 272: 12116-21. PMID: 9115281
  12. The p38-MAPK inhibitor, SB203580, inhibits cardiac stress-activated protein kinases/c-Jun N-terminal kinases (SAPKs/JNKs).  |  Clerk, A. and Sugden, PH. 1998. FEBS Lett. 426: 93-6. PMID: 9598985
  13. SB 203580 inhibits p38 mitogen-activated protein kinase, nitric oxide production, and inducible nitric oxide synthase in bovine cartilage-derived chondrocytes.  |  Badger, AM., et al. 1998. J Immunol. 161: 467-73. PMID: 9647257

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

SB 203580, 1 mg

sc-3533
1 mg
$88.00

SB 203580, 5 mg

sc-3533A
5 mg
$342.00

It's in powder form, how do I reconstitute this ?? Thank you :)) 어디에 수화하면 될까요..? 감사합니다!

Asked by: toothless dragon
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2025-08-11

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. The compound, sc-3533, is provided as a white powder. If you have any further the questions or concerns, please feel free to contact our Technical Services department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-17

What is the long-term storage recommendation for this product in solution with DMSO?

Asked by: two2igm05
Thank you for your question. In DMSO, it can be stored for up to 6 months without degradation at -80 degrees Celsius. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-16
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Rated 5 out of 5 by from 挺好的前几天刚买的,感觉还不错!实验结果和预想的一致,但我一直没搞明白,SB203580能否抑制p38MAPK的磷酸化?
Date published: 2020-08-22
Rated 5 out of 5 by from SB203580I bough this copped to study its effects on ipsc. Im very happy with the compound performance.
Date published: 2018-03-19
Rated 5 out of 5 by from Good features!I bought it 28 months ago and boght it again 25 months ago. The inhibitive effect was very good and specific.
Date published: 2016-11-01
Rated 5 out of 5 by from The SCBT productThe SCBT product, sc-3533, a p38MAPK inhibitor, was used to pre-treat HUVEC cells prior to exposure to TNF in an effort to see if TNF has an effect on genes involved in p38MAPK mediated down regulation (PubMed ID 24299952). -SCBT Publication Review
Date published: 2015-03-06
Rated 5 out of 5 by from H1299 and A549 cells were preH1299 and A549 cells were pre-treated with sc-3533 (from SCBT) before exposure to LY2603618 to see if p38 MAPK plays a part in LY2603618 induced caspase cleavage (PubMed ID 24928205). -SCBT Publication Review
Date published: 2015-03-06
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SB 203580 | CAS 152121-47-6 is rated 5.0 out of 5 by 5.
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