Date published: 2025-12-16

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Ionomycin (CAS 56092-82-1)

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Alternate Names:
Ionomycin Calcium Salt
Application:
Ionomycin is a reversible ion binding calcium ionophore, polyether antibiotic, and apoptotic degeneration inducer
CAS Number:
56092-82-1
Purity:
≥98%
Molecular Weight:
747.07
Molecular Formula:
C41H70O9•Ca
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Ionomycin is a calcium ionophore widely utilized in biological research due to its effect on intracellular calcium levels by facilitating the transport of Ca2+ across cell membranes. It is employed in studies to understand the role of calcium as a secondary messenger in cellular signaling pathways. Because of its specific and potent ionophoretic activity, ionomycin is useful in experiments designed to elucidate the mechanisms of calcium-dependent processes, such as muscle contraction, neurotransmitter release, and gene expression. Researchers also use ionomycin to investigate the activation of calcium-dependent enzymes and the subsequent effects on various physiological functions. Its ability to induce apoptosis in certain cell types is another area of study, as this process is often regulated by intracellular calcium concentrations.


Ionomycin (CAS 56092-82-1) References

  1. Phosphatidic acid as the biosynthetic precursor of the endocannabinoid 2-arachidonoylglycerol in intact mouse neuroblastoma cells stimulated with ionomycin.  |  Bisogno, T., et al. 1999. J Neurochem. 72: 2113-9. PMID: 10217292
  2. Stress protein GRP78 prevents apoptosis induced by calcium ionophore, ionomycin, but not by glycosylation inhibitor, tunicamycin, in human prostate cancer cells.  |  Miyake, H., et al. 2000. J Cell Biochem. 77: 396-408. PMID: 10760948
  3. Influences of ionomycin, dibutyryl-cycloAMP and tumour necrosis factor-alpha on intracellular amount and secretion of apM1 in differentiating primary human preadipocytes.  |  Kappes, A. and Löffler, G. 2000. Horm Metab Res. 32: 548-54. PMID: 11246823
  4. Activation of stress response by ionomycin in rat hepatoma cells.  |  Sreedhar, AS. and Srinivas, UK. 2002. J Cell Biochem. 86: 154-61. PMID: 12112026
  5. Actions of ionomycin, 4-BrA23187 and a novel electrogenic Ca2+ ionophore on mitochondria in intact cells.  |  Abramov, AY. and Duchen, MR. 2003. Cell Calcium. 33: 101-12. PMID: 12531186
  6. Ionomycin downregulates beta-catenin/Tcf signaling in colon cancer cell line.  |  Park, CH., et al. 2005. Carcinogenesis. 26: 1929-33. PMID: 15930030
  7. Demonstration of a calcium requirement for secretory protein processing and export. Differential effects of calcium and dithiothreitol.  |  Kuznetsov, G., et al. 1992. J Biol Chem. 267: 3932-9. PMID: 1740441
  8. Liberation of [3H]arachidonic acid and changes in cytosolic free calcium in fura-2-loaded human platelets stimulated by ionomycin and collagen.  |  Pollock, WK., et al. 1986. Biochem J. 235: 869-77. PMID: 3092807
  9. Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin.  |  Locke, M., et al. 2020. Sci Rep. 10: 11694. PMID: 32678135
  10. Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria.  |  Kauffman, RF., et al. 1980. J Biol Chem. 255: 2735-9. PMID: 6766939
  11. Ca2+ ionophore-induced apoptosis on cultured embryonic rat cortical neurons.  |  Takei, N. and Endo, Y. 1994. Brain Res. 652: 65-70. PMID: 7525020
  12. Ionomycin, thapsigargin, ryanodine, and sperm induced Ca2+ release increase during meiotic maturation of mouse oocytes.  |  Jones, KT., et al. 1995. J Biol Chem. 270: 6671-7. PMID: 7896808
  13. Potentiation of calcium- and caffeine-induced calcium release by cyclic ADP-ribose.  |  Lee, HC. 1993. J Biol Chem. 268: 293-9. PMID: 8416936
  14. Contrasting effect of transforming growth factor type beta 1 (TGF-beta 1) on proliferation and interleukin-2 receptor expression in activated and rapidly cycling immature (CD3-CD4-CD8-) thymocytes.  |  Dupuy d'Angeac, A., et al. 1993. J Cell Physiol. 154: 44-52. PMID: 8419406
  15. Alterations in Bcl-2/Bax protein levels in platelets form part of an ionomycin-induced process that resembles apoptosis.  |  Vanags, DM., et al. 1997. Br J Haematol. 99: 824-31. PMID: 9432028

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ionomycin, 1 mg

sc-3592
1 mg
$76.00

Ionomycin, 5 mg

sc-3592A
5 mg
$265.00

What is the working concentration in cell culture

Asked by: rhian22
Thank you for contacting Santa Cruz Biotechnology. Ionomycin can be obtained as either the free acid or the Ca2+ salt. Br-A23187 is available as the free acid. All forms are soluble in dry DMSO, which can be used to prepare stock solutions. Because these ionophores are very hydrophobic, they are bound avidly by serum proteins. Serum proteins such as BSA, when present in the medium, greatly reduce the effectiveness of ionophores and, if possible, should be left out of the experimental medium when ionophores are to be used. Much higher concentrations of ionophore must be used. Br-A23187 and ionomycin have been used at concentrations ranging from 10− 7 to 10− 5 M.
Answered by: BlakeJ
Date published: 2024-05-07

What is the Country of Origin?

Asked by: SCM4
This product originates in Israel.
Answered by: Tech Service 11
Date published: 2017-03-17

What is the expected color of this product?

Asked by: chemicalsmg
Thank you for your question. Ionomycin: sc-3592 is expected to be white to off-white in color.
Answered by: Chemical Support 3
Date published: 2017-03-10
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Rated 5 out of 5 by from worked for flow cytometry stimulation!worked for flow cytometry stimulation! and happy about it
Date published: 2019-12-04
Rated 5 out of 5 by from Use this routinely in the lab and works wellUse this routinely in the lab and works well.
Date published: 2015-08-12
Rated 5 out of 5 by from No commentNo comment.
Date published: 2015-04-06
Rated 5 out of 5 by from Commonly used in research that is focusedCommonly used in research that is focused on calcium transport across membranes. Used to stimulate whole human blood, then cells were stained with antibodies against CD69 and IFN- to show its affect. A decrease in the levels of IFN- was seen, and an increase in expression of CD69 (looks to be a small increase) by flow cytometry. -SCBT QC
Date published: 2015-02-13
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Ionomycin is rated 5.0 out of 5 by 4.
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