PMA CAS: 16561-29-8
MF: C36H56O8
MW: 616.83

PMA (CAS 16561-29-8)

PMA | CAS 16561-29-8 is rated 4.8 out of 5 by 6.
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Alternate Names: PMA is also known as Phorbol-12-Myristate-13-Acetate.
Application: PMA is a reversible and highly potent protein kinase C (PKC) activator in vitro and in vivo at nM concentrations.
CAS Number: 16561-29-8
Purity: ≥95%
Molecular Weight: 616.83
Molecular Formula: C36H56O8
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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PMA (Phorbol-12-Myristate-13-Acetate) is a diester of phorbol and is a tumor promoting compound extracted from croton oil. PMA is a reversible, highly potent protein kinase C (PKC) activator in vitro and in vivo at nM concentrations. PMA′s effects on PKC are attributed to its similarity to diacylgylerol, a natural activator of PKC. PMA activates Ca2+-ATPase and potentiates forskolin-induced cAMP formation. PMA has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells. PMA is also known as Phorbol-12-Myristate-13-Acetate, TPA, 12-O-Tetradecanoylphorbol-13-acetate, PKC Activator I, and tetradecanoic acid, (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-9a-(acetyloxy)-1a,1b,4,4a,5,7a,7b,8,9,9a-decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9-yl ester.


References

1. Castagna, M., et al. 1982. J. Biol. Chem. 257: 7847-7851. PMID: 7085651

2. Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. U.S.A. 82: 2774-2778. PMID: 2859591

3. Saltis, J., et al. 1991. J. Biol. Chem. 266: 261-267. PMID: 1985898

4. Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73: 2011-2015. PMID: 1474079

5. Oishi, K. and Yamaguchi, M. 1994. J. Cell. Biochem. 55: 168-172. PMID: 8089192

6. Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. U.S.A. 92: 8443-8447. PMID: 7545303

7. Powell, D.J., et al. 2003. Mol. Cell. Biol. 23: 7794-7808. PMID: 14560023

8. Saraiva, L., et al. 2004. Eur. J. Pharmacol. 491: 101-110. PMID: 15140626

9. Shimura, C., et al. 2010. Am. J. Pathol. 176: 227-237. PMID: 20008150

Usage :
Stable for up to 1 year after receipt when stored at -20°C. Stock solutions should be aliquoted and stored at -20°C for up to 1 month.
Physical State :
Solid
Solubility :
Soluble in DMSO (25 mg/ml), 100%ethanol (25 mg/ml), acetone, ether, DMF, methanol, and chloroform. Insoluble in water, and aqueous buffers.
Storage :
Store at -20° C
Melting Point :
50-70° C
Boiling Point :
~698.10° C at 760 mmHg (Predicted)
Density :
1.17 g/cm3 (Predicted)
Refractive Index :
n20D 1.55 (Predicted)
Optical Activity :
α24D +49, c = 1 in dioxane
Ki Data :
PKC: Ki= 2.6 nM
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
QH4377000
PubChem CID :
Merck Index :
14: 7332
MDL Number :
MFCD00036736
Beilstein Registry :
2407201
SMILES :

Download SDS (MSDS)

Certificate of Analysis

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PMA (CAS 16561-29-8)  Product Citations

See how others have used PMA (CAS 16561-29-8). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 95 total

PMID: # 34192597  Pazoki, H.|Mohammad Rahimi, H.|Mirjalali, H.|Niyyati, M.|Mosaffa, N.|Seyed Tabaei, SJ.|Shahrokh, S.|Asadzadeh Aghdaei, H.|Zali, MR.| et al. 2021. Microb Pathog. 158: 105072.

PMID: # 34044082  Pap, N.|Reshamwala, D.|Korpinen, R.|Kilpeläinen, P.|Fidelis, M.|Furtado, MM.|Sant'Ana, AS.|Wen, M.|Zhang, L.|Hellström, J.|Marnilla, P.|Mattila, P.|Sarjala, T.|Yang, B.|Lima, ADS.|Azevedo, L.|Marjomäki, V.|Granato, D.| et al. 2021. Food Chem Toxicol. 153: 112284.

PMID: # 33558623  Malek, N.|Michrowska, A.|Mazurkiewicz, E.|Mrówczyńska, E.|Mackiewicz, P.|Mazur, AJ.| et al. 2021. Sci Rep. 11: 3329.

PMID: # 34408919  Shibata, H.|Xu, N.|Saito, S.|Zhou, L.|Ozgenc, I.|Webb, J.|Fu, C.|Zolkind, P.|Egloff, AM.|Uppaluri, R.| et al. 2021. Oncoimmunology. 10: 1958589.

PMID: # 34063504  Damato, M.|Cardon, T.|Wisztorski, M.|Fournier, I.|Pieragostino, D.|Cicalini, I.|Salzet, M.|Vergara, D.|Maffia, M.| et al. 2021. Int J Mol Sci. 22:

PMID: # 33894488  Dziedzic, K.|Węgrzyn, P.|Gałęzowski, M.|Bońkowska, M.|Grycuk, K.|Satała, G.|Wiatrowska, K.|Wiklik, K.|Brzózka, K.|Nowak, M.| et al. 2021. Int Immunopharmacol. 96: 107645.

PMID: # 34161786  Liang, D.|Cheloha, RW.|Watanabe, T.|Gardella, TJ.|Baskin, JM.| et al. 2021. Cell Chem Biol.

PMID: # 33622708  Chan, W.|Gottschalk, RA.|Yao, Y.|Pomerantz, JL.|Germain, RN.| et al. 2021. Immunohorizons. 5: 117-132.

PMID: # 34453672  Nemati, S.|Pazoki, H.|Mohammad Rahimi, H.|Asadzadeh Aghdaei, H.|Shahrokh, S.|Baghaei, K.|Mirjalali, H.|Zali, MR.| et al. 2021. Mol Biol Rep.

PMID: # 34324891  Prins, MMC.|van Roest, M.|Vermeulen, JLM.|Tjabringa, GS.|van de Graaf, SFJ.|Koelink, PJ.|Wildenberg, ME.| et al. 2021. J Immunol Methods. 497: 113106.

Citations 1 to 10 of 95 total

I would like to dissolve the PMA ( code no 16561-29-8) in DMSO to use it in tissue culture, but DMSO is toxic to cells and the final concentration should be less than ).1% , so what is the concentration of DMSO used to dissolve the stock PMA??

Asked by: Alubaidi
Thank you for your question. PMA is soluble in DMSO (25 mg/ml), 100% ethanol (25 mg/ml), acetone, ether, DMF, methanol, and chloroform. Since it is insoluble in water and aqueous buffers, tissue culture use of this product may in fact prove difficult.
Answered by: Tech Support Europe
Date published: 2021-02-03
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Rated 5 out of 5 by from work well Using PMA from Santa to active neutrophils to NETosis and it works well as the same product from Sigma
Date published: 2018-01-15
Rated 5 out of 5 by from Great PKC activator Ised this PKC activator many times with great results.
Date published: 2017-07-05
Rated 5 out of 5 by from well done We had used PMA for a long time(final conentration 10nmol-100nmol), PMA-treated THP-1 cells grew well and stable, and Western blot worked well too!
Date published: 2016-11-01
Rated 4 out of 5 by from thp-1 differentiate into macrophages thp-1 cells can differentiate into macrophages by using PMA.
Date published: 2016-11-01
Rated 5 out of 5 by from An increase in c An increase in c-Fos protein expression was observed (nuclear extracts) by WB in Jurkat cells post treatment with PMA. Other proteins affected by treatment with PMA include: p-PKC , caspase-6, p63 . -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Zhou et Zhou et. al. (PubMed ID 23174781) used PMA to mimic Resistin to activate NF- B (via PKC - PKG signal transduction pathway). -SCBT QC
Date published: 2015-02-01
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