FAM153B Activators
Chemical activators of FAM153B can initiate a cascade of intracellular events leading to its phosphorylation and subsequent activation. Forskolin, a diterpene found in the Indian Coleus plant, directly stimulates adenylate cyclase, thereby increasing intracellular levels of cAMP. This elevation in cAMP can activate protein kinase A (PKA), a key regulator of phosphorylation for various proteins, which in turn can target FAM153B for phosphorylation. Similarly, 8-Bromo-cAMP and Dibutyryl-cAMP, both analogs of cAMP, permeate cellular membranes and engage PKA to phosphorylate target proteins, including FAM153B. The activation of PKA through these cAMP analogs ensures that FAM153B is modified appropriately within the signaling pathways.
Other activators function by manipulating intracellular calcium levels, which is also crucial for protein phosphorylation. Compounds such as Ionomycin and A23187 (Calcimycin) act as calcium ionophores, increasing the cytosolic concentration of calcium, which can activate calmodulin-dependent kinase (CaMK). This kinase can phosphorylate FAM153B as part of its downstream signaling. Thapsigargin, by inhibiting the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), causes a similar rise in cytosolic calcium, indirectly promoting the activation of FAM153B. In addition, BAY K8644, which acts as an L-type calcium channel agonist, leads to an influx of calcium, potentially resulting in the activation of FAM153B through calcium-dependent signaling pathways. The synthetic DAG analog 1,2-Dioctanoyl-sn-glycerol (DiC8) activates protein kinase C (PKC), which is implicated in phosphorylating proteins including FAM153B. Furthermore, Phorbol 12-myristate 13-acetate (PMA) is a known PKC activator, thereby contributing to the phosphorylation landscape of FAM153B. Lastly, inhibitors of protein phosphatases such as Calyculin A and Okadaic Acid prevent dephosphorylation, maintaining FAM153B in a phosphorylated state by inhibiting phosphatases PP1 and PP2A. This mechanism ensures that once FAM153B has been phosphorylated, it remains active for a prolonged period.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which is involved in the phosphorylation of many protein targets. PKC activation can lead to the phosphorylation of FAM153B as part of its downstream signaling. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin acts as a calcium ionophore, increasing intracellular calcium concentration. Elevated calcium can activate calmodulin-dependent kinase (CaMK), which could phosphorylate FAM153B as part of its downstream signaling processes. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $126.00 $328.00 | 30 | |
8-Bromo-cAMP is a cell-permeable cAMP analog that activates cAMP-dependent protein kinases (PKA). PKA can phosphorylate various substrates, potentially including FAM153B, thus leading to its activation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to an increase in cytosolic calcium levels, which can activate calcium-dependent proteins and potentially result in the activation of FAM153B through downstream signaling effects. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A). Inhibition of these phosphatases can lead to increased phosphorylation levels of proteins, including FAM153B, as it may prevent the dephosphorylation of FAM153B, maintaining it in an active state. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases PP1 and PP2A, similar to Calyculin A. By inhibiting dephosphorylation, it can indirectly maintain the phosphorylation and thus activation state of proteins such as FAM153B. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is another cell-permeable analog of cAMP that activates PKA. Activation of PKA leads to phosphorylation of various proteins, and through this pathway, dibutyryl-cAMP can lead to the activation of FAM153B. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 acts as a calcium ionophore similarly to Ionomycin, raising intracellular calcium levels. Increased calcium can activate numerous calcium-dependent proteins, which may include kinases that could phosphorylate and activate FAM153B. | ||||||
(±)-Bay K 8644 | 71145-03-4 | sc-203324 sc-203324A sc-203324B | 1 mg 5 mg 50 mg | $84.00 $196.00 $817.00 | ||
BAY K8644 is an L-type calcium channel agonist, which leads to increased calcium influx. The resulting elevation in intracellular calcium can activate signaling pathways, including those involving kinases that can phosphorylate and thereby activate FAM153B. | ||||||
1,2-Dioctanoyl-sn-glycerol | 60514-48-9 | sc-202397 sc-202397A | 10 mg 50 mg | $47.00 $254.00 | 2 | |
DiC8 is a synthetic analog of diacylglycerol (DAG) and serves as an activator of PKC. Since PKC is involved in phosphorylation events within the cell, DiC8 can facilitate the phosphorylation and consequent activation of FAM153B. | ||||||