Date published: 2025-11-21

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Dibutyryl-cAMP (CAS 16980-89-5)

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Alternate Names:
Dibutyryl-cAMP is also known as 4H-Furo[3,2-d]-1,3,2-dioxaphosphorin, Adenosine deriv.; Bucladesine Sodium; Bucladesine Sodium Salt; DC 2797; Dibutyryl 3′,5′-Cyclic AMP Sodium Salt; Dibutyryl Cyclic AMP Monosodium Salt; Dibutyryl Cyclic AMP Sodium; Dibutyryl Cyclic AMP Sodium Salt; Dibutyryl-cAMP Sodium Salt; Monosodium Dibutyryl Cyclic AMP; N-6,O-2′-Dibutyryladenosine Cyclic 3′,5′-Monophosphate Sodium Salt; N6,2′-O-Dibutyryl-3′,5′-cyclic AMP Sodium Salt
Application:
Dibutyryl-cAMP is a cell-permable analog of cyclic AMP (cAMP) that stimulates cAMP-dependent protein kinases.
CAS Number:
16980-89-5
Purity:
≥98%
Molecular Weight:
491.37
Molecular Formula:
C18H23N5O8P•Na
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Dibutyryl-cAMP is an analog of cyclic AMP that stimulates PKA (cAMP-dependent protein kinase). Dibutyryl-cAMP has been shown not to effect cAMP levels, gap junction communication or cumulus expansion in cumulus-oocyte complexes. Research shows that dibutyryl-cAMP can stimulate the activity of NaK+ ATPase (Na+K+ -pump), bring about morphological differentiation of astrocytes, and block contraction of vas deferens in rat studies. Additionally, other studies show that Dibutyryl-cAMP can upregulate Nur77 expression through modification of histones during neurite outgrowth in PC-12 cells, and regulate the mRNA levels of Dp71 and dystrophin in rat brain astrocytes.


Dibutyryl-cAMP (CAS 16980-89-5) References

  1. Regulation of Na(+)-K(+)-Cl(-) cotransporter in primary astrocytes by dibutyryl cAMP and high [K(+)](o).  |  Su, G., et al. 2000. Am J Physiol Cell Physiol. 279: C1710-21. PMID: 11078685
  2. Dibutyryl-cAMP (dbcAMP) up-regulates astrocytic chloride-dependent L-[3H]glutamate transport and expression of both system xc(-) subunits.  |  Gochenauer, GE. and Robinson, MB. 2001. J Neurochem. 78: 276-86. PMID: 11461963
  3. Selective modulation of lipopolysaccharide-stimulated cytokine expression and mitogen-activated protein kinase pathways by dibutyryl-cAMP in BV2 microglial cells.  |  Woo, MS., et al. 2003. Brain Res Mol Brain Res. 113: 86-96. PMID: 12750010
  4. PKA-dependent activation of PKC, p38 MAPK and IKK in macrophage: implication in the induction of inducible nitric oxide synthase and interleukin-6 by dibutyryl cAMP.  |  Chio, CC., et al. 2004. Cell Signal. 16: 565-75. PMID: 14751542
  5. Effect of dibutyryl cAMP on the cAMP content, meiotic progression, and developmental potential of in vitro matured pre-pubertal and adult pig oocytes.  |  Bagg, MA., et al. 2006. Mol Reprod Dev. 73: 1326-32. PMID: 16865720
  6. Lack of correlation between activation of cyclic AMP-dependent protein kinase and inhibition of contraction of rat vas deferens by cyclic AMP analogs.  |  Hei, YJ., et al. 1991. Mol Pharmacol. 39: 233-8. PMID: 1847496
  7. Neural stem/progenitor cells differentiate in vitro to neurons by the combined action of dibutyryl cAMP and interferon-gamma.  |  Zahir, T., et al. 2009. Stem Cells Dev. 18: 1423-32. PMID: 19355840
  8. Effect of follicle size and dibutyryl cAMP on the cAMP content and gap junctional communication of porcine prepubertal cumulus-oocyte complexes during IVM.  |  Bagg, MA., et al. 2009. Reprod Fertil Dev. 21: 796-804. PMID: 19567222
  9. Dibutyryl-cAMP up-regulates nur77 expression via histone modification during neurite outgrowth in PC12 cells.  |  Maruoka, H., et al. 2010. J Biochem. 148: 93-101. PMID: 20375114
  10. HeLa human cervical cancer cell migration is inhibited by treatment with dibutyryl-cAMP.  |  Lee, JW., et al. 2014. Anticancer Res. 34: 3447-55. PMID: 24982353
  11. Dibutyryl-cAMP attenuates pulmonary fibrosis by blocking myofibroblast differentiation via PKA/CREB/CBP signaling in rats with silicosis.  |  Liu, Y., et al. 2017. Respir Res. 18: 38. PMID: 28222740
  12. Turnover of regulatory subunit of cyclic AMP-dependent protein kinase in S49 mouse lymphoma cells. Regulation by catalytic subunit and analogs of cyclic AMP.  |  Steinberg, RA. and Agard, DA. 1981. J Biol Chem. 256: 10731-4. PMID: 6270127
  13. Apoptosis of vascular smooth muscle cells. Protein kinase C and oncoprotein Bcl-2 are involved in regulation of apoptosis in non-transformed rat vascular smooth muscle cells.  |  Leszczynski, D., et al. 1994. Am J Pathol. 145: 1265-70. PMID: 7527616
  14. Differential expression of dystrophin isoforms and utrophin during dibutyryl-cAMP-induced morphological differentiation of rat brain astrocytes.  |  Imamura, M. and Ozawa, E. 1998. Proc Natl Acad Sci U S A. 95: 6139-44. PMID: 9600931
  15. Protein kinase A induces recruitment of active Na+,K+-ATPase units to the plasma membrane of rat proximal convoluted tubule cells.  |  Carranza, ML., et al. 1998. J Physiol. 511 (Pt 1): 235-43. PMID: 9679177

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Dibutyryl-cAMP, 20 mg

sc-201567
20 mg
$45.00

Dibutyryl-cAMP, 100 mg

sc-201567A
100 mg
$130.00

Dibutyryl-cAMP, 500 mg

sc-201567B
500 mg
$480.00

Dibutyryl-cAMP, 10 g

sc-201567C
10 g
$4450.00

How long is it stable in -20ºC after reconstitution in H2O at 200mM?

Asked by: DLopez
Thank you for your question. We recommend keeping the compound at -20 °C for long-term storage. We do not have viability data on long-term storage in solution.
Answered by: Tech Support Europe
Date published: 2024-06-07

How long is it stable in -20C after reconstitution in DMSO?

Asked by: DCAMP Stability
Thank you for your question. The solution should be stored at –20 C, protected from light and will be stable for 3-6 months. However, we highly recommend our customers to make fresh solution prior to their experiments for quality purposes and freeze/thaw cycles should be minimized.
Answered by: BlakeJ
Date published: 2024-04-09

Will the powder degrade if stored at room temperature?

Asked by: IsaK
Thank you for your question. Dibutyryl-cAMP (CAS 16980-89-5) should always be stored at -20 °C. The compound will lose its viability if stored at room temperature for a prolonged period of time. Solutions of the product should be prepared fresh before use.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Do you have any recommendations for stability once the product is in solution? Should freeze/thaw cycles be minimized?

Asked by: two2igm05
Thank you for your question. The solution should be stored at –20 C, protected from light and will be stable for 3-6 months. However, we highly recommend making fresh solution prior to their experiments for quality purposes and freeze/thaw cycles should be minimized. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-18
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Rated 5 out of 5 by from Dibutyryl-cAMP to differentiate NG108 cellsI use Dibutyryl-cAMP to differentiate a neuroblastoma-glioma hybrid cell line, NG108. It is working well by now. The cells exhibit neuron-like morphology.
Date published: 2023-02-28
Rated 5 out of 5 by from Great features!Excellent activator of PKA for use in HEK293 cells.
Date published: 2022-06-07
Rated 5 out of 5 by from Good chemicals, best serviceI bought this chemical about a month ago, and used it immediately. Chemical works as expected and it came overnight shipping. thanks
Date published: 2020-03-09
Rated 5 out of 5 by from Incredible speedWhenever I had a question I always got an answer in a few hours. The ordered product was delivered in a day.
Date published: 2019-11-21
Rated 5 out of 5 by from Used in cell cultureUsed in cell culture. Satisfied with product.
Date published: 2015-10-07
Rated 5 out of 5 by from Used it in our experiments and happy with purchaseUsed it in our experiments and happy with purchase.
Date published: 2015-06-24
Rated 5 out of 5 by from A decrease in p27 protein expression wasA decrease in p27 protein expression was observed by WB in SH-SY5Y cells post treatment with Dibutyryl-cAMP. -SCBT QC
Date published: 2015-03-16
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Dibutyryl-cAMP is rated 5.0 out of 5 by 7.
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