Shank 2 Antibody (B-4): sc-365121

Shank 2 Antibody (B-4) is a mouse monoclonal IgG2b antibody that detects Shank 2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Shank 2 is a crucial component of the postsynaptic density, serving as a molecular scaffold that organizes and stabilizes synaptic proteins, which is vital for proper synaptic function and plasticity. Shank 2 plays a significant role in anchoring glutamate receptors and other signaling molecules at excitatory synapses, thereby facilitating effective neurotransmission. The structure of Shank 2 includes multiple protein-protein interaction domains, such as PDZ domains, which are essential for its function in synaptic signaling. These domains allow Shank 2 to interact with various proteins, including ion channels and signaling enzymes, ensuring the precise localization of these molecules at the synapse. The proper functioning of Shank 2 is critical for maintaining synaptic integrity and is implicated in various neurodevelopmental disorders when dysregulated. Anti-Shank 2 antibody (B-4) is an invaluable tool for researchers studying synaptic biology and the molecular mechanisms underlying synaptic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Shank 2 Antibody (B-4) References:

1. Shank2 mutant mice display a hypersecretory response to cholera toxin.  |  Jung, ES., et al. 2014. J Physiol. 592: 1809-21. PMID: 24445315
2. A critical role of spinal Shank2 proteins in NMDA-induced pain hypersensitivity.  |  Yoon, SY., et al. 2017. Mol Pain. 13: 1744806916688902. PMID: 28326932
3. Deficiency of Shank2 causes mania-like behavior that responds to mood stabilizers.  |  Pappas, AL., et al. 2017. JCI Insight. 2: PMID: 29046483
4. SHANK2 mutations associated with autism spectrum disorder cause hyperconnectivity of human neurons.  |  Zaslavsky, K., et al. 2019. Nat Neurosci. 22: 556-564. PMID: 30911184
5. SHANK2 is a frequently amplified oncogene with evolutionarily conserved roles in regulating Hippo signaling.  |  Xu, L., et al. 2021. Protein Cell. 12: 174-193. PMID: 32661924
6. SHANK2 mutations impair apoptosis, proliferation and neurite outgrowth during early neuronal differentiation in SH-SY5Y cells.  |  Unsicker, C., et al. 2021. Sci Rep. 11: 2128. PMID: 33483523
7. Enhanced fear limits behavioral flexibility in Shank2-deficient mice.  |  Yun, M., et al. 2022. Mol Autism. 13: 40. PMID: 36192805
8. Shank2 identifies a subset of glycinergic neurons involved in altered nociception in an autism model.  |  Olde Heuvel, F., et al. 2023. Mol Autism. 14: 21. PMID: 37316943
9. Sex-specific modulation of safety learning in Shank2-deficient mice.  |  Kreutzmann, JC., et al. 2024. Prog Neuropsychopharmacol Biol Psychiatry. 132: 110973. PMID: 38369099
10. Differential effectiveness of dietary zinc supplementation with autism-related behaviours in Shank2 knockout mice.  |  Lee, K., et al. 2024. Philos Trans R Soc Lond B Biol Sci. 379: 20230230. PMID: 38853567

The preferred Shank 2 antibody is Shank 2 Antibody (A-11): sc-271834.