Shank 2 Antibody (A-11): sc-271834

Shank 2 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Shank 2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Shank 2 antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Shank 2 is a critical component of the postsynaptic density (PSD), a specialized structure that plays a vital role in synaptic signaling and plasticity. The PSD is essential for anchoring and clustering glutamate receptors, which are crucial for excitatory neurotransmission. Shank 2 contains multiple protein interaction domains, including PDZ domains, which facilitate the assembly of signaling complexes by recognizing specific sequence motifs at the C-terminus of target proteins. This interaction is fundamental for maintaining synaptic integrity and function, as Shank 2 helps coordinate the localization of ion channels and receptors, thereby influencing synaptic strength and plasticity. Shank 2′s ability to interact with other proteins, such as betaPIX and PAK, underscores Shank 2′s role in regulating the structural dynamics of dendritic spines, which are critical for synaptic transmission and cognitive processes. The diverse splice variants of Shank proteins enhance the complexity of Shank 2 interactions within the PSD, ensuring proper neuronal development and function.

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Shank 2 Antibody (A-11) References:

1. Characterization of the Shank family of synaptic proteins. Multiple genes, alternative splicing, and differential expression in brain and development.  |  Lim, S., et al. 1999. J Biol Chem. 274: 29510-8. PMID: 10506216
2. The Shank family of scaffold proteins.  |  Sheng, M. and Kim, E. 2000. J Cell Sci. 113 (Pt 11): 1851-6. PMID: 10806096
3. The G protein-coupled receptor CL1 interacts directly with proteins of the Shank family.  |  Tobaben, S., et al. 2000. J Biol Chem. 275: 36204-10. PMID: 10958799
4. Regulation of dendritic spine morphology and synaptic function by Shank and Homer.  |  Sala, C., et al. 2001. Neuron. 31: 115-30. PMID: 11498055
5. ProSAP/Shank proteins - a family of higher order organizing molecules of the postsynaptic density with an emerging role in human neurological disease.  |  Boeckers, TM., et al. 2002. J Neurochem. 81: 903-10. PMID: 12065602
6. The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42.  |  Park, E., et al. 2003. J Biol Chem. 278: 19220-9. PMID: 12626503
7. Shank 2 expression coincides with neuronal differentiation in the developing retina.  |  Kim, JH., et al. 2009. Exp Mol Med. 41: 236-42. PMID: 19299912
8. Cerebellar Shank2 Regulates Excitatory Synapse Density, Motor Coordination, and Specific Repetitive and Anxiety-Like Behaviors.  |  Ha, S., et al. 2016. J Neurosci. 36: 12129-12143. PMID: 27903723
9. Cell-Type-Specific Shank2 Deletion in Mice Leads to Differential Synaptic and Behavioral Phenotypes.  |  Kim, R., et al. 2018. J Neurosci. 38: 4076-4092. PMID: 29572432
10. Distinct Phenotypes of Shank2 Mouse Models Reflect Neuropsychiatric Spectrum Disorders of Human Patients With SHANK2 Variants.  |  Eltokhi, A., et al. 2018. Front Mol Neurosci. 11: 240. PMID: 30072871
11. Effect of the autism-associated lncRNA Shank2-AS on architecture and growth of neurons.  |  Luo, T., et al. 2019. J Cell Biochem. 120: 1754-1762. PMID: 30160788
12. Phenotypic spectrum of SHANK2-related neurodevelopmental disorder.  |  Caumes, R., et al. 2020. Eur J Med Genet. 63: 104072. PMID: 32987185

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Shank 2 Antibody (A-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Shank 2 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Shank 2 Antibody (A-11) Specifications

Alternate Product Name: SHANK2

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 996-1110

Epitope Target: Shank 2

Epitope Species: human

Product Reactivity/Detection: Shank 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Shank 2 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin