UBCE7IP4 Activators
Chemical activators of UBCE7IP4 engage distinct cellular mechanisms to modulate the protein's activity. Forskolin, by increasing intracellular cyclic AMP levels, indirectly activates Protein Kinase A, which in turn can initiate a phosphorylation cascade affecting various substrates, including those involved in the pathway of UBCE7IP4. This series of events can lead to the activation of UBCE7IP4. Similarly, Ionomycin raises intracellular calcium levels, which in turn activates calmodulin-dependent kinases capable of phosphorylating proteins within UBCE7IP4-related pathways. Phorbol 12-myristate 13-acetate, commonly known as PMA, targets protein kinase C, triggering phosphorylation events that can culminate in UBCE7IP4 activation. Thapsigargin, by disrupting calcium homeostasis through the inhibition of the SERCA pump, causes an increase in cytosolic calcium, which activates calcium-dependent kinases that may phosphorylate and activate UBCE7IP4.
Another approach involves the use of Okadaic Acid and Calyculin A, both of which inhibit protein phosphatases PP1 and PP2A. The inhibition leads to a reduced dephosphorylation rate of proteins within the UBCE7IP4 signaling network, effectively maintaining UBCE7IP4 in an activated state due to higher phosphorylation levels. Oppositely, KN-93, by inhibiting Ca2+/calmodulin-dependent protein kinase II, can induce the activation of UBCE7IP4 through alternative kinases that compensate for the inhibition. LY294002 disrupts PI3K signaling, altering downstream AKT signaling and potentially leading to the activation of kinases that phosphorylate and activate UBCE7IP4. In a similar vein, PD 98059 and U0126, both targeting MEK, can result in the activation of UBCE7IP4 due to activation of parallel signaling pathways or feedback loops. Lastly, the calcium ionophore A23187 increases intracellular calcium, which could activate kinases that phosphorylate components in UBCE7IP4's pathway, culminating in the activation of the protein. H-89, despite being a PKA inhibitor, can lead to the activation of UBCE7IP4 through the induction of compensatory pathways that activate the protein.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylate cyclase, leading to an increase in cyclic AMP (cAMP) levels. Elevated cAMP activates PKA (Protein Kinase A), which can phosphorylate a range of substrates that may include components within the signaling pathways involving UBCE7IP4, resulting in its activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calmodulin-dependent kinases. These kinases can then phosphorylate proteins involved in the UBCE7IP4-regulated pathways, thereby activating UBCE7IP4. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which is known to phosphorylate a broad range of target proteins. PKC-mediated phosphorylation within the signaling pathways that UBCE7IP4 is a part of can lead to the functional activation of UBCE7IP4. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the Sarco/Endoplasmic Reticulum Ca2+-ATPase (SERCA), leading to an increase in cytosolic calcium levels, which activates calcium-dependent kinases. These kinases could phosphorylate members of the signaling cascade that includes UBCE7IP4, leading to its activation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases PP1 and PP2A. Inhibition of these phosphatases can lead to increased phosphorylation levels of proteins in the signaling pathways that involve UBCE7IP4, effectively activating UBCE7IP4. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Similar to Okadaic Acid, Calyculin A is an inhibitor of protein phosphatases, particularly PP1 and PP2A. By inhibiting these phosphatases, Calyculin A causes an increase in the phosphorylation state of proteins that are involved in the signaling networks of UBCE7IP4, which leads to the activation of UBCE7IP4. | ||||||
KN-93 | 139298-40-1 | sc-202199 | 1 mg | $178.00 | 25 | |
KN-93 is an inhibitor of Ca2+/calmodulin-dependent protein kinase II (CaMKII). By inhibiting CaMKII, KN-93 could lead to compensatory activation of alternative kinases that may phosphorylate and hence activate UBCE7IP4. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is an inhibitor of phosphoinositide 3-kinases (PI3K). By inhibiting PI3K, LY294002 can alter downstream AKT signaling, which may result in the activation of kinases that phosphorylate substrates involved in UBCE7IP4's pathway, leading to the activation of UBCE7IP4. | ||||||
PD 98059 | 167869-21-8 | sc-3532 sc-3532A | 1 mg 5 mg | $39.00 $90.00 | 212 | |
PD 98059 is an inhibitor of MEK, which is upstream of ERK in the MAPK pathway. Inhibition of MEK may lead to the activation of parallel signaling pathways or compensatory feedback loops that involve the functional activation of UBCE7IP4. | ||||||
U-0126 | 109511-58-2 | sc-222395 sc-222395A | 1 mg 5 mg | $63.00 $241.00 | 136 | |
U0126 is another MEK inhibitor, like PD 98059, and it may also cause the activation of alternative pathways or feedback mechanisms that result in the functional activation of UBCE7IP4. | ||||||