PACS-1b Inhibitors
Chemical inhibitors of PACS-1b function through various mechanisms that disrupt the protein's sorting and trafficking capabilities within the cell. Wortmannin, for instance, targets phosphoinositide 3-kinases (PI3Ks), essential for vesicle formation, and by inhibiting PI3Ks, it prevents the proper functioning of PACS-1b in vesicle trafficking. Dyngo-4a and MiTMAB are dynamin inhibitors; they impede the scission of clathrin-coated vesicles, essential for endocytosis and exocytosis processes that PACS-1b regulates. Pitstop 2, another inhibitor, specifically blocks clathrin-mediated endocytosis by preventing the interaction between clathrin and its adaptor proteins, which are crucial for the formation of clathrin-coated vesicles and hence PACS-1b's role in these processes. Similarly, Exo1 disrupts the exocyst complex, which is key for vesicle tethering to the plasma membrane, a step necessary for the exocytic pathway where PACS-1b is involved.
On the other hand, Brefeldin A disrupts the Golgi apparatus structure, directly affecting the PACS-1b-associated protein sorting at the trans-Golgi network. Golgicide A specifically inhibits the Golgi BFA resistance factor 1 (GBF1), leading to a cessation of ARF-mediated vesicular trafficking from the Golgi, which is another pathway where PACS-1b is crucial. ML141, an inhibitor of the Cdc42 GTPase, along with EHOp-016 and CID 1067700 that target Rac1 GTPase and ARHGAP21 respectively, disrupt the organization of the actin cytoskeleton. This organization is foundational for vesicle movement, which PACS-1b depends on for its function. Finally, SecinH3 and SMIFH2 inhibit cytohesins and the formin family of proteins, which are involved in actin polymerization and cytoskeletal reorganization. The disruption of vesicular transport processes resulting from these inhibitions affects the sorting and trafficking functions of PACS-1b, showcasing the broad spectrum of mechanisms that chemical inhibitors can employ to modulate the function of this protein.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $66.00 $219.00 $417.00 | 97 | |
Wortmannin is a potent inhibitor of phosphoinositide 3-kinases (PI3Ks). PI3Ks are involved in various signaling pathways that can regulate trafficking and sorting processes mediated by PACS-1b. Inhibiting PI3K can disrupt vesicle formation and trafficking, leading to functional inhibition of PACS-1b. | ||||||
Hydroxy-Dynasore | 1256493-34-1 | sc-364678 | 10 mg | $250.00 | ||
Dyngo-4a inhibits dynamin, a GTPase involved in the scission of clathrin-coated vesicles from the membrane. Since PACS-1b is implicated in trafficking associated with the trans-Golgi network and endosomes, inhibition of dynamin can disrupt the endocytic pathway and consequently inhibit PACS-1b function. | ||||||
Pitstop 2 | 1419093-54-1 | sc-507418 | 10 mg | $360.00 | ||
Pitstop 2 is a selective inhibitor of clathrin-mediated endocytosis. By blocking the interaction between clathrin and its adaptor proteins, it can impede the formation of clathrin-coated vesicles. This action can inhibit the endocytic trafficking routes that involve PACS-1b, effectively inhibiting its function. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $30.00 $52.00 $122.00 $367.00 | 25 | |
Brefeldin A disrupts the structure and function of the Golgi apparatus. As PACS-1b is involved in protein sorting at the trans-Golgi network, the disruption of Golgi function by this inhibitor would directly inhibit the protein sorting function of PACS-1b. | ||||||
Exo1 | 461681-88-9 | sc-200752 sc-200752A | 10 mg 50 mg | $82.00 $291.00 | 4 | |
Exo1 inhibits the exocyst complex, which is involved in the tethering of vesicles to the plasma membrane for secretion. Since PACS-1b is implicated in protein sorting and trafficking, disrupting exocyst function can inhibit the exocytic pathway and indirectly inhibit PACS-1b function. | ||||||
ML 141 | 71203-35-5 | sc-362768 sc-362768A | 5 mg 25 mg | $134.00 $502.00 | 7 | |
ML141 is a potent and selective inhibitor of the Cdc42 GTPase. Cdc42 is involved in actin cytoskeleton organization, which is important for vesicle trafficking. Inhibition of Cdc42 can disrupt vesicular transport processes potentially involved with PACS-1b, leading to its functional inhibition. | ||||||
SecinH3 | 853625-60-2 | sc-203260 | 5 mg | $273.00 | 6 | |
SecinH3 inhibits cytohesins, which are guanine nucleotide exchange factors for ARF GTPases. Since ARF GTPases are involved in vesicle formation and trafficking, SecinH3 can inhibit ARF-mediated pathways and indirectly inhibit the vesicular transport functions of PACS-1b. | ||||||
Golgicide A | 1005036-73-6 | sc-215103 sc-215103A | 5 mg 25 mg | $187.00 $670.00 | 11 | |
Golgicide A is a specific inhibitor of the Golgi BFA resistance factor 1 (GBF1), a GEF for ARF GTPase. By inhibiting GBF1, it disrupts ARF-mediated vesicular trafficking from the Golgi, thus inhibiting PACS-1b's function in protein sorting and trafficking. | ||||||
SMIFH2 | 340316-62-3 | sc-507273 | 5 mg | $140.00 | ||
SMIFH2 is an inhibitor of the formin family of proteins, which are involved in actin polymerization and cytoskeletal reorganization. Because vesicle trafficking relies on the cytoskeleton, inhibiting formin can disrupt vesicular transport and indirectly inhibit PACS-1b function. | ||||||
EHop-016 | 1380432-32-5 | sc-497382 | 5 mg | $78.00 | ||
EHOp-016 is a selective inhibitor of the Rac1 GTPase. Rac1 is involved in actin cytoskeleton rearrangement, which is crucial for vesicle movement and docking. Inhibition of Rac1 can therefore disrupt processes critical to PACS-1b-related trafficking and sorting. | ||||||