NHE-6 Activators
Chemical activators of NHE-6 can enhance its function through various cellular mechanisms. EIPA, known primarily as an inhibitor of Na+/H+ exchangers, can indirectly increase the activity of NHE-6 by inhibiting other isoforms of the exchanger, which may cause a compensatory upregulation of NHE-6 activity to maintain ion balance within the cell. Zinc, as an allosteric modulator, can bind to specific sites on NHE-6, inducing a conformational change that enhances its transporter activity. Similarly, polyamines such as spermine and spermidine may interact with NHE-6 at polyamine binding sites, potentially leading to an increase in its activity. Forskolin, by elevating intracellular cAMP levels, activates protein kinase A (PKA), which could phosphorylate NHE-6 if it is within PKA's substrate range, thereby increasing its activity. IBMX raises cAMP levels by inhibiting phosphodiesterases, leading to a similar activation of PKA and potential phosphorylation of NHE-6.
Dibutyryl-cAMP, a cAMP analog, can directly activate PKA, which in turn may phosphorylate and activate NHE-6. Phorbol esters like PMA activate protein kinase C (PKC), which could also phosphorylate NHE-6 if it is a PKC substrate. Calcium ionophores such as ionomycin and A23187 increase intracellular calcium levels, which could activate calmodulin-dependent kinases. These kinases may target NHE-6, leading to its activation through phosphorylation. Calyculin A and okadaic acid, as inhibitors of protein phosphatases, prevent the dephosphorylation of proteins. If NHE-6 is regulated by phosphorylation, the inhibition of these phosphatases would result in sustained activation of NHE-6 due to increased phosphorylation levels. Each of these chemicals can influence the activity of NHE-6 by modulating the cell's biochemical pathways, ensuring that NHE-6 is functionally active.
SEE ALSO...
Items 1 to 10 of 11 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Zinc | 7440-66-6 | sc-213177 | 100 g | $47.00 | ||
Zinc ions can act as allosteric modulators of several enzymes and transporters. If NHE-6 has allosteric sites responsive to zinc, the binding of zinc could lead to a conformational change that increases NHE-6 activity. | ||||||
Spermine | 71-44-3 | sc-212953A sc-212953 sc-212953B sc-212953C | 1 g 5 g 25 g 100 g | $60.00 $192.00 $272.00 $883.00 | 1 | |
As a polyamine, spermine can interact with various ion channels and transporters. If NHE-6 has binding sites for polyamines, spermine could bind and induce a structural change that activates NHE-6. | ||||||
Spermidine | 124-20-9 | sc-215900 sc-215900B sc-215900A | 1 g 25 g 5 g | $56.00 $595.00 $173.00 | ||
Similar to spermine, spermidine is a polyamine that could interact with NHE-6 if the protein has polyamine binding sites, potentially leading to an increase in transporter activity. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylate cyclase, increasing cAMP levels. Elevated cAMP can activate PKA, which may phosphorylate NHE-6, leading to increased activity if NHE-6 is a substrate for PKA. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
IBMX increases intracellular cAMP by inhibiting phosphodiesterases. Higher cAMP levels can enhance PKA activity, which could phosphorylate and activate NHE-6 if it is a target for PKA. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
db-cAMP is a cell-permeable cAMP analog that activates PKA. PKA activation could lead to phosphorylation and activation of NHE-6 if it is a PKA substrate. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC). If NHE-6 is a substrate for PKC, activation of PKC by PMA could lead to phosphorylation and subsequent activation of NHE-6. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calmodulin-dependent kinases, which may phosphorylate and activate NHE-6 if it is responsive to this pathway. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is another calcium ionophore that can raise intracellular calcium levels, potentially activating calcium-dependent kinases that could phosphorylate and activate NHE-6. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatases 1 and 2A. By inhibiting these phosphatases, calyculin A could prevent dephosphorylation of NHE-6, resulting in its sustained activation. | ||||||