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IBMX (CAS 28822-58-4)

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Alternate Names:
IBMX also known as 3-Isobutyl-1-methylanxthine; Methylisobutylxanthine; MIX; 1H-Purine-2,6-dione, 3,7-dihydro-1-methyl-3-(2-methylpropyl)-; 3,7-dihydro-1-methyl-3-(2-methylpropyl)-1h-purine-6-dione; 3-Isobutyl-1-methyl-3,7-dihydro-1H-purine-2,6-dione;3-Isobutyl-1-methylanxthine; 3-isobutyl-1-methyl-xanthin; IMX
Application:
IBMX is a potent and nonspecific inhibitor of PDE (phosphodiesterases) and can increase intracellular cyclic AMP levels
CAS Number:
28822-58-4
Purity:
≥99%
Molecular Weight:
222.24
Molecular Formula:
C10H14N4O2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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IBMX is one of the most potent and nonspecific inhibitors of cyclic nucleotide PDE (phosphodiesterases) with documented IC50 values for PDE1, 2, 3, 4, and 5. It is documented to raise intracellular cyclic AMP levels. In rat sensory neurons, the addition of IBMX caused the release of Ca2+ from caffeine ryanodine sensitive internal stores increasing the internal Ca2+ levels and inducing a biphasic membrane current response. Through PDE inhibition, IBMX has also inhibited TNFalpha. IBMX in breast cancer cells has also induced p21 and p27 through a PKA independent pathway. Although a nonspecific PDE inhibitor, IBMX does not show inhibition of PDE8B. In neuroendocrine epithelial cells, IBMX has been observed to inhibit alpha-adrenoceptor-mediated 5-HT release at IC50 = 1.3muM.


IBMX (CAS 28822-58-4) References

  1. Effects of IBMX on norepinephrine-induced vasoconstriction in small mesenteric arteries.  |  Taylor, MS., et al. 1999. Am J Physiol. 276: G909-14. PMID: 10198334
  2. The biphasic induction of p21 and p27 in breast cancer cells by modulators of cAMP is posttranscriptionally regulated and independent of the PKA pathway.  |  Rao, S., et al. 1999. Exp Cell Res. 252: 211-23. PMID: 10502413
  3. 3-isobutyl-1-methylxanthine (IBMX) sensitizes cardiac myocytes to anoxia.  |  Geisbuhler, TP., et al. 2002. Biochem Pharmacol. 63: 2055-62. PMID: 12093483
  4. Fsk and IBMX inhibit proliferation and proapoptotic of glioma stem cells via activation of cAMP signaling pathway.  |  Lv, P., et al. 2019. J Cell Biochem. 120: 321-331. PMID: 30171713
  5. Forskolin and IBMX Induce Neural Transdifferentiation of MSCs Through Downregulation of the NRSF.  |  Thompson, R., et al. 2019. Sci Rep. 9: 2969. PMID: 30814572
  6. Combined use of dbcAMP and IBMX minimizes the damage induced by a long-term artificial meiotic arrest in mouse germinal vesicle oocytes.  |  Wu, XC., et al. 2020. Mol Reprod Dev. 87: 262-273. PMID: 31943463
  7. Inhibition of germinal vesicle breakdown using IBMX increases microRNA-21 in the porcine oocyte.  |  Hale, BJ., et al. 2020. Reprod Biol Endocrinol. 18: 39. PMID: 32393269
  8. The Phosphodiesterase Inhibitor IBMX Blocks the Potassium Channel THIK-1 from the Extracellular Side.  |  Zou, X., et al. 2020. Mol Pharmacol. 98: 143-155. PMID: 32616523
  9. Cyclic adenosine monophosphate (cAMP) analog and phosphodiesterase inhibitor (IBMX) ameliorate human sperm capacitation and motility.  |  Piroozmanesh, H., et al. 2022. Rev Int Androl. 20 Suppl 1: S24-S30. PMID: 35811239
  10. Effects of xanthine derivatives on lipolysis and on adenosine 3',5'-monophosphate phosphodiesterase activity.  |  Beavo, JA., et al. 1970. Mol Pharmacol. 6: 597-603. PMID: 4322367
  11. 3-Isobutyl-1-methylxanthine (IBMX) affects potassium permeability in rat sensory neurones via pathways that are sensitive and insensitive to [Ca2+]in.  |  Usachev, Y., et al. 1995. Pflugers Arch. 430: 420-8. PMID: 7491267
  12. IBMX induces calcium release from intracellular stores in rat sensory neurones.  |  Usachev, Y. and Verkhratsky, A. 1995. Cell Calcium. 17: 197-206. PMID: 7542569
  13. Phosphodiesterase inhibitors suppress alpha2-adrenoceptor-mediated 5-hydroxytryptamine release from tracheae of newborn rabbits.  |  Freitag, A., et al. 1998. Eur J Pharmacol. 354: 67-71. PMID: 9726632

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

IBMX, 200 mg

sc-201188
200 mg
$159.00

IBMX, 500 mg

sc-201188B
500 mg
$315.00

IBMX, 1 g

sc-201188A
1 g
$598.00

What is the expected appearance of this product?

Asked by: chemicalsmg
Thank you for your question. IBMX: sc-201188 is expected to be a white to off-white solid.
Answered by: Chemical Support 3
Date published: 2017-04-05

What is the appropriate temperature when you say it will dissolve in DMSO with gentle warming?

Asked by: SCchem16
Thank you for your question. IBMX, sc-201188, can be dissolved in DMSO warmed to ~37ºC.
Answered by: TechService7
Date published: 2017-02-14
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Rated 5 out of 5 by from Freitag et alFreitag et al. (PubMed ID 9726632) used IBMX to regulate cyclic nucleotide mediated inhibition of 5-HT release from neuroendocrine cells by inhibiting phosphodiesterase. -SCBT Publication Review
Date published: 2015-05-31
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IBMX is rated 5.0 out of 5 by 1.
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