CRISP-2 Inhibitors
Chemical inhibitors of CRISP-2 can function through various mechanisms to disrupt the protein's role in sperm-egg fusion. Calcium Ionophore A23187, by increasing intracellular calcium levels, can disrupt the delicate balance of calcium that is critical for sperm activation, a process in which CRISP-2 is intimately involved. The disruption of normal calcium signaling can impede CRISP-2's facilitation of sperm-egg fusion. Similarly, BAPTA-AM, by chelating intracellular calcium, can hinder the calcium-dependent processes essential for the acrosome reaction, a necessary precursor to the fusion that CRISP-2 helps mediate. NF449, as an antagonist of the P2X1 receptor, disrupts calcium signaling pathways, potentially affecting the acrosome reaction and the role of CRISP-2 in this process. U73122 can inhibit phospholipase C, thereby affecting the generation of second messengers like diacylglycerol and inositol trisphosphate, which are pivotal for sperm activation and the subsequent actions of CRISP-2.
Further, the chemical inhibitors targeting kinases have distinct roles in impeding CRISP-2's function. PD168393 and Genistein can inhibit tyrosine kinase-mediated pathways, which could otherwise influence sperm capacitation and the acrosome reaction, thereby affecting CRISP-2. LY294002 and Wortmannin, as PI3K inhibitors, suppress downstream signaling that could intersect with CRISP-2's role in sperm maturation and function. Go6983 and KT5823, which inhibit PKC and PKG respectively, can alter the phosphorylation state of proteins involved in sperm motility and capacitation, thereby indirectly hindering CRISP-2's function in these processes. ZM447439, by inhibiting Aurora kinases, can alter cell cycle regulation and potentially impact processes that prepare sperm for fertilization where CRISP-2 is involved. Lastly, ML-7 inhibits myosin light chain kinase, potentially affecting sperm motility and the acrosome reaction, both of which are processes where CRISP-2 is essential.
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Items 1 to 10 of 11 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
This compound increases intracellular calcium levels, which is critical for sperm activation and the acrosome reaction, processes that CRISP-2 is involved in. By disrupting normal calcium signaling, this inhibitor could impede CRISP-2's function in promoting sperm-egg fusion. | ||||||
PD 168393 | 194423-15-9 | sc-222138 | 1 mg | $162.00 | 4 | |
An irreversible inhibitor of the EGFR kinase which is involved in signal transduction pathways that could intersect with CRISP-2's role in sperm maturation and motility. Inhibiting EGFR could disrupt signaling required for CRISP-2 to exert its function in fertilization. | ||||||
Gö 6983 | 133053-19-7 | sc-203432 sc-203432A sc-203432B | 1 mg 5 mg 10 mg | $103.00 $293.00 $465.00 | 15 | |
A pan-PKC inhibitor that can disrupt protein kinase C activity, which is implicated in sperm motility and the acrosome reaction. As CRISP-2 is involved in these processes, inhibition of PKC can hinder the functional role of CRISP-2 in sperm cells. | ||||||
BAPTA/AM | 126150-97-8 | sc-202488 sc-202488A | 25 mg 100 mg | $138.00 $449.00 | 61 | |
A cell-permeant calcium chelator that can diminish intracellular calcium levels, potentially impairing the calcium-dependent processes in which CRISP-2 participates, such as the acrosome reaction and sperm-egg fusion. | ||||||
Genistein | 446-72-0 | sc-3515 sc-3515A sc-3515B sc-3515C sc-3515D sc-3515E sc-3515F | 100 mg 500 mg 1 g 5 g 10 g 25 g 100 g | $26.00 $92.00 $120.00 $310.00 $500.00 $908.00 $1821.00 | 46 | |
A tyrosine kinase inhibitor that can interfere with signal transduction pathways influencing sperm capacitation and acrosome reaction, thereby potentially impairing CRISP-2's function in fertilization. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
A PI3K inhibitor that can suppress the PI3K/Akt signaling pathway, which is involved in a variety of cellular processes including those that could be necessary for CRISP-2's role in sperm maturation and function. | ||||||
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $66.00 $219.00 $417.00 | 97 | |
Another PI3K inhibitor that can reduce PI3K activity, impacting signaling pathways that are potentially involved in CRISP-2-mediated sperm-egg interaction. | ||||||
NF449 | 627034-85-9 | sc-478179 sc-478179A sc-478179B | 10 mg 25 mg 100 mg | $199.00 $460.00 $1479.00 | 1 | |
A potent and selective antagonist of the P2X1 receptor, which is involved in calcium signaling that can be pivotal for the acrosome reaction where CRISP-2 has functional relevance. | ||||||
KT5823 | 126643-37-6 | sc-3534 sc-3534A sc-3534B | 100 µg 1 mg 5 mg | $178.00 $1204.00 $4799.00 | 21 | |
A selective inhibitor of protein kinase G (PKG), which is involved in sperm capacitation; as CRISP-2 is a part of the capacitation process, inhibiting PKG could inhibit CRISP-2's function. | ||||||
ZM-447439 | 331771-20-1 | sc-200696 sc-200696A | 1 mg 10 mg | $150.00 $349.00 | 15 | |
An Aurora kinase inhibitor that can disrupt cell cycle regulation and potentially impact sperm maturation, indirectly affecting the functional role of CRISP-2 in the process of fertilization. | ||||||