A530082C11Rik Activators
Chemical activators of solute carrier family 35, member E2 (SLC35E2) have diverse mechanisms of action, each initiating a cascade of intracellular events that culminate in the phosphorylation and activation of this protein. Sodium orthovanadate, for example, exerts its effect by activating protein tyrosine phosphatases, which in turn maintain the phosphorylation state of SLC35E2, crucial for its optimal activity. Forskolin, on the other hand, elevates cyclic AMP (cAMP) levels through adenylate cyclase activation, leading to the activation of protein kinase A (PKA). PKA then phosphorylates SLC35E2, enhancing its transport function within cellular membranes. Similarly, phorbol 12-myristate 13-acetate (PMA) triggers the activation of protein kinase C, which can directly phosphorylate SLC35E2, potentially increasing its functional capacity.
Furthermore, agents that modulate intracellular calcium levels, such as A23187 (Calcimycin), ionomycin, thapsigargin, and Bay K8644, activate calcium-dependent kinases. These kinases can phosphorylate SLC35E2, thereby amplifying its transport capabilities. A23187 and ionomycin function as ionophores, directly increasing calcium influx, while thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), and Bay K8644 selectively activates L-type calcium channels, both leading to elevated intracellular calcium levels. Additionally, Brefeldin A disrupts the Golgi structure and induces a cellular stress response that can lead to the phosphorylation of SLC35E2. MG-132 prevents proteasomal degradation, allowing for the accumulation of kinases that target SLC35E2. Tunicamycin, by inhibiting N-linked glycosylation, triggers cellular stress that can result in the activation of kinases that phosphorylate SLC35E2. Finally, monensin disrupts ion gradients and activates sodium-sensitive kinases, which then target SLC35E2. Similarly, SB 203580, primarily a p38 MAP kinase inhibitor, can activate alternative pathways that result in the phosphorylation and activation of SLC35E2. Each of these chemicals, through their unique interactions within the cell, contribute to the regulation and activation of SLC35E2, playing a role in its function as a transport protein.
SEE ALSO...
Items 1 to 10 of 11 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $45.00 $56.00 $183.00 | 142 | |
Sodium orthovanadate activates protein tyrosine phosphatases, which can lead to the activation of solute carrier family 35, member E2 by maintaining its phosphorylation state, essential for its function in nucleotide-sugar transport. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) activates Protein Kinase C, which could phosphorylate and thereby activate solute carrier family 35, member E2, enhancing its transport efficiency within the Golgi apparatus. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 (Calcimycin) increases intracellular calcium levels which can activate calcium-dependent kinases that could phosphorylate and enhance the activity of solute carrier family 35, member E2, promoting its transport function. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin acts as an ionophore for calcium, elevating intracellular calcium levels which can lead to the activation of calmodulin-dependent kinases that phosphorylate and activate solute carrier family 35, member E2 to increase its transport capabilities. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to increased intracellular calcium levels, which activate kinases that may phosphorylate and activate solute carrier family 35, member E2, enhancing its function. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $30.00 $52.00 $122.00 $367.00 | 25 | |
Brefeldin A disrupts Golgi structure, which can induce a cellular stress response, leading to the activation of kinases that could phosphorylate solute carrier family 35, member E2, thereby increasing its transport activity. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG-132 inhibits proteasomal degradation, which could lead to the accumulation of regulatory proteins that activate kinases involved in the phosphorylation and activation of solute carrier family 35, member E2, thus enhancing its transport function. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $169.00 $299.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation, causing cellular stress that can lead to the activation of stress kinases which may phosphorylate and activate solute carrier family 35, member E2, crucial for maintaining glycosylation transport functions. | ||||||
Monensin A | 17090-79-8 | sc-362032 sc-362032A | 5 mg 25 mg | $152.00 $515.00 | ||
Monensin acts as an ionophore for sodium and disrupts ion gradients, this can activate sodium-sensitive kinases that phosphorylate and activate solute carrier family 35, member E2, thereby increasing its nucleotide-sugar transport activity. | ||||||
(±)-Bay K 8644 | 71145-03-4 | sc-203324 sc-203324A sc-203324B | 1 mg 5 mg 50 mg | $82.00 $192.00 $801.00 | ||
Bay K8644 selectively activates L-type calcium channels, leading to increased intracellular calcium levels which activate kinases that phosphorylate and activate solute carrier family 35, member E2, enhancing its transport activity within the cell. | ||||||