Topo IIα Antibody (G-6): sc-166934
- Topo IIα Antibody (G-6) is a mouse monoclonal IgG2b κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1301-1531 of Topo IIα of human origin
- Topo II alpha Antibody (G-6) is recommended for detection of Topo IIα of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Topo II alpha Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Topo IIα Antibody (G-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Topo IIα Antibody (G-6) (see ordering information below).
QUICK LINKS
Topo IIα Antibody (G-6) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect DNA topoisomerase II alpha (Topo IIα), also known as topoisomerase IIα. Anti-Topo IIα antibody (G-6) targets an epitope encompassing amino acids 1301-1531 of the human Topo IIα protein, ensuring precise binding and reliable performance in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). DNA topoisomerase II alpha is a crucial nuclear enzyme composed of two identical subunits, each featuring distinct domains including the N-terminal ATPase domain, the core DNA-binding domain, and the C-terminal regulatory domain. The intricate structure of Topo IIα enables management of DNA topology by introducing transient double-strand breaks, facilitating essential cellular processes like DNA replication, transcription, chromosome condensation, and segregation. This structural complexity is vital for maintaining genomic stability and ensuring accurate cell division, making Topo IIα a significant target for various anticancer therapies. Topo IIα monoclonal antibody (G-6) demonstrates robust reactivity with human Topo IIα, allowing researchers to effectively study the enzyme′s structural dynamics and functional roles within the cell. Additionally, Topo IIα exists alongside its isoform Topo IIβ, with each isoform displaying distinct structural features and cellular functions, highlighting the specificity and importance of Topo IIα (G-6) antibody in differentiating between these closely related proteins. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Topo IIα (G-6) antibody provides versatile tools for diverse experimental setups. With high affinity and specificity, Topo IIα (G-6) monoclonal antibody facilitates accurate detection and quantification of Topo IIα, advancing research into DNA topology, cell cycle regulation, and the development of topoisomerase-targeting therapeutics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Topo IIα Antibody (G-6) | sc-166934 | 200 µg/ml | $316.00 | |||
Topo IIα Antibody (G-6): m-IgG Fc BP-HRP Bundle | sc-529089 | 200 µg Ab; 10 µg BP | $354.00 | |||
Topo IIα Antibody (G-6): m-IgGκ BP-HRP Bundle | sc-521805 | 200 µg Ab, 40 µg BP | $354.00 | |||
Topo IIα Antibody (G-6): m-IgG2b BP-HRP Bundle | sc-550080 | 200 µg Ab; 10 µg BP | $354.00 | |||
Topo IIα Antibody (G-6) AC | sc-166934 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Topo IIα Antibody (G-6) HRP | sc-166934 HRP | 200 µg/ml | $316.00 | |||
Topo IIα Antibody (G-6) FITC | sc-166934 FITC | 200 µg/ml | $330.00 | |||
Topo IIα Antibody (G-6) PE | sc-166934 PE | 200 µg/ml | $343.00 | |||
Topo IIα Antibody (G-6) Alexa Fluor® 488 | sc-166934 AF488 | 200 µg/ml | $357.00 | |||
Topo IIα Antibody (G-6) Alexa Fluor® 546 | sc-166934 AF546 | 200 µg/ml | $357.00 | |||
Topo IIα Antibody (G-6) Alexa Fluor® 594 | sc-166934 AF594 | 200 µg/ml | $357.00 | |||
Topo IIα Antibody (G-6) Alexa Fluor® 647 | sc-166934 AF647 | 200 µg/ml | $357.00 | |||
Topo IIα Antibody (G-6) Alexa Fluor® 680 | sc-166934 AF680 | 200 µg/ml | $357.00 | |||
Topo IIα Antibody (G-6) Alexa Fluor® 790 | sc-166934 AF790 | 200 µg/ml | $357.00 |