Topo IIα Antibody (F-12): sc-365916
- Topo IIα Antibody (F-12) is a mouse monoclonal IgG1 κ Topo IIα antibody, cited in 25 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1511-1530 at the C-terminus of Topo IIα of human origin
- Topo II alpha Antibody (F-12) is recommended for detection of Topo IIα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including porcine
- Anti-Topo II alpha Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Topo IIα Antibody (F-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Topo IIα Antibody (F-12) (see ordering information below).
QUICK LINKS
Topo IIα Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the Topoisomerase II alpha (Topo IIα) protein, also known as DNA Topoisomerase II alpha or TOP2A. anti-Topo IIα antibody (F-12) recognizes an epitope mapped between amino acids 1511-1530 at the C-terminus of human Topo IIα, ensuring precise binding and detection. Topo IIα is a crucial nuclear enzyme involved in the regulation of DNA topology during processes such as replication, transcription, and chromosome segregation. Topo IIα interacts with several key proteins, including BRCA1, which plays a role in DNA repair, and p53, a vital tumor suppressor protein, highlighting its importance in maintaining genomic stability. anti-Topo IIα antibody (F-12) exhibits broad species reactivity, effectively recognizing Topo IIα from mouse, rat, human, and additional species like porcine, making it versatile for various experimental applications. Topo IIα monoclonal antibody (F-12) is compatible with multiple laboratory techniques, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated and conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-Topo IIα antibody (F-12) offers flexibility for diverse research needs. By facilitating the transient breaking and rejoining of DNA strands, Topo IIα ensures proper chromosomal architecture and function, making anti-Topo IIα antibody (F-12) an essential tool for scientists studying cellular processes and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Topo IIα Antibody (F-12) References:
- Topoisomerase II alpha and topoisomerase II beta genes: characterization and mapping to human chromosomes 17 and 3, respectively. | Tan, KB., et al. 1992. Cancer Res. 52: 231-4. PMID: 1309226
- Structure of the human type I DNA topoisomerase gene. | Kunze, N., et al. 1991. J Biol Chem. 266: 9610-6. PMID: 1851751
- Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells. | Austin, CA. and Fisher, LM. 1990. FEBS Lett. 266: 115-7. PMID: 2163884
- Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes. | Chung, TD., et al. 1989. Proc Natl Acad Sci U S A. 86: 9431-5. PMID: 2556712
- cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment. | D'Arpa, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 2543-7. PMID: 2833744
- The mechanisms of DNA topoisomerases. | Roca, J. 1995. Trends Biochem Sci. 20: 156-60. PMID: 7770916
- A model for the mechanism of human topoisomerase I. | Stewart, L., et al. 1998. Science. 279: 1534-41. PMID: 9488652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Topo IIα Antibody (F-12) | sc-365916 | 200 µg/ml | $322.00 | |||
Topo IIα Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-529510 | 200 µg Ab; 10 µg BP | $361.00 | |||
Topo IIα Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-522420 | 200 µg Ab, 40 µg BP | $361.00 | |||
Topo IIα Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-543552 | 200 µg Ab; 20 µg BP | $361.00 | |||
Topo IIα Antibody (F-12) AC | sc-365916 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Topo IIα Antibody (F-12) HRP | sc-365916 HRP | 200 µg/ml | $322.00 | |||
Topo IIα Antibody (F-12) FITC | sc-365916 FITC | 200 µg/ml | $336.00 | |||
Topo IIα Antibody (F-12) PE | sc-365916 PE | 200 µg/ml | $349.00 | |||
Topo IIα Antibody (F-12) Alexa Fluor® 488 | sc-365916 AF488 | 200 µg/ml | $364.00 | |||
Topo IIα Antibody (F-12) Alexa Fluor® 546 | sc-365916 AF546 | 200 µg/ml | $364.00 | |||
Topo IIα Antibody (F-12) Alexa Fluor® 594 | sc-365916 AF594 | 200 µg/ml | $364.00 | |||
Topo IIα Antibody (F-12) Alexa Fluor® 647 | sc-365916 AF647 | 200 µg/ml | $364.00 | |||
Topo IIα Antibody (F-12) Alexa Fluor® 680 | sc-365916 AF680 | 200 µg/ml | $364.00 | |||
Topo IIα Antibody (F-12) Alexa Fluor® 790 | sc-365916 AF790 | 200 µg/ml | $364.00 | |||
Topo IIα (F-12) Neutralizing Peptide | sc-365916 P | 100 µg/0.5 ml | $69.00 |