TLK1 Activators
TLK1 Activators are a collection of chemical entities that bolster the kinase activity of TLK1 through a variety of intricate cellular mechanisms. ATP, being the primary substrate for TLK1's kinase action, directly augments its activity by providing the phosphate groups for TLK1's phosphorylation reactions essential for DNA replication and repair processes. Magnesium and zinc ions, supplied by magnesium chloride and zinc sulfate respectively, act as crucial cofactors and allosteric modulators, enhancing the structural stability of TLK1 and thus its enzymatic function in chromatin assembly. Sodium orthovanadate and okadaic acid, through their roles as phosphatase inhibitors, indirectly maintain the phosphorylation status of TLK1's substrates, promoting the protein's signaling pathways especially in cell cycle regulation and DNA damage response. Caffeine, by modulating the ATM/ATR pathway, and forskolin, by raising intracellular cAMP levels, indirectly heighten TLK1's activity, which is vital for the maintenance of genomic integrity.
Further indirect activators include 5-iodotubercidin and dibutyryl-cAMP, which enhance the availability of ATP, a requisite for TLK1's kinase activity, and activate PKA, a kinase that can foster a cellular milieu conducive to TLK1 function within DNA damage response pathways. Staurosporine, in low concentrations, can paradoxically engender a selective activation of kinases like TLK1 by modulating the phosphorylation landscape within the cell. LY294002, by inhibiting PI3K, may alleviate inhibitory phosphorylations on TLK1, thus facilitating the kinase's activity. Finally, UCN-01, as a protein kinase C inhibitor, has the potential to augment TLK1's role in cell cycle checkpoints by decreasing inhibitory phosphorylations on TLK1 or its associated substrates, thereby ensuring the proper signaling for cellular responses to genotoxic stress. Collectively, these TLK1 Activators enhance the protein's activity through their targeted and synergistic effects on cellular signaling pathways.
Items 1 to 10 of 12 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
ADP | 58-64-0 | sc-507362 | 5 g | $54.00 | ||
ATP serves as a direct substrate for TLK1, providing the phosphate groups necessary for TLK1's kinase activity. Phosphorylation of substrate proteins by TLK1 leads to enhanced functional activity related to DNA repair and replication processes. | ||||||
Magnesium chloride | 7786-30-3 | sc-255260C sc-255260B sc-255260 sc-255260A | 10 g 25 g 100 g 500 g | $28.00 $35.00 $48.00 $125.00 | 2 | |
Magnesium ion is a cofactor necessary for the kinase activity of TLK1, facilitating the transfer of phosphate groups to substrate proteins, thus enhancing TLK1's activity in cellular responses to DNA damage. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc ions act as allosteric modulators of TLK1, stabilizing its structure and thus enhancing its kinase activity which is crucial for the phosphorylation of proteins involved in chromatin assembly. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $49.00 $57.00 $187.00 | 142 | |
Acts as a phosphatase inhibitor, preventing dephosphorylation of proteins, including TLK1 substrates, thus indirectly enhancing the TLK1 signaling cascade involved in cell cycle regulation. | ||||||
Caffeine | 58-08-2 | sc-202514 sc-202514A sc-202514B sc-202514C sc-202514D | 50 g 100 g 250 g 1 kg 5 kg | $33.00 $67.00 $97.00 $192.00 $775.00 | 13 | |
Caffeine is an atypical activator of the ATM/ATR pathway, which TLK1 is a part of; it enhances TLK1 kinase activity through the stabilization of the complex, thus promoting the repair of DNA double-strand breaks. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
As a potent inhibitor of protein phosphatases PP1 and PP2A, okadaic acid indirectly increases the phosphorylation state of TLK1 substrates, leading to enhanced TLK1-mediated signaling in DNA damage response. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Through the elevation of intracellular cAMP levels and subsequent activation of PKA, forskolin can enhance TLK1 kinase activity indirectly by creating a favorable cellular environment for TLK1's role in the cell cycle. | ||||||
5-Iodotubercidin | 24386-93-4 | sc-3531 sc-3531A | 1 mg 5 mg | $153.00 $464.00 | 20 | |
A potent adenosine kinase inhibitor that could increase the availability of ATP in cells, indirectly enhancing the activity of ATP-dependent enzymes like TLK1, which require ATP for their kinase activity. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $153.00 $396.00 | 113 | |
Although a broad-spectrum kinase inhibitor, at low concentrations staurosporine can selectively activate certain kinases; it may result in the selective enhancement of TLK1 pathways by altering the phosphorylation dynamics within the cell. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
A PI3K inhibitor that can lead to alterations in AKT signaling, potentially freeing TLK1 from inhibitory phosphorylation by AKT-mediated pathways, thereby enhancing TLK1 kinase activity. | ||||||