TLK1 Antibody (G-8): sc-393515
- TLK1 Antibody (G-8) is a mouse monoclonal IgG2a κ TLK1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 126-161 within an internal region of TLK1 of human origin
- TLK1 Antibody (G-8) is recommended for detection of TLK1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-TLK1 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TLK1 Antibody (G-8) for WB applications. These reagents are now offered in bundles with TLK1 Antibody (G-8) (see ordering information below).
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TLK1 Antibody (G-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TLK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TLK1 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Tousled-like kinases, TLK1 and TLK2, are essential serine/threonine kinases that play a critical role in cell cycle regulation and organ morphogenesis, with their activity peaking during the S phase of the cell cycle. TLK1 is predominantly localized in the nucleus, where TLK1 is involved in the phosphorylation of key substrates that facilitate DNA replication and repair processes. This nuclear localization is crucial as TLK1 effectively regulates the phosphorylation of proteins involved in chromatin remodeling and DNA damage response, thereby ensuring genomic stability and proper cellular function. Additionally, TLK1 exhibits a tendency to dimerize through interactions between coiled-coil regions, which enhances enzymatic activity and regulatory functions. TLK1 expression is particularly high in mouse testis, especially in pachytene spermatocytes and round spermatids, highlighting TLK1′s significant role in spermatogenesis and the development of multicellular organisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TLK1 Antibody (G-8) References:
- Tlk, a novel evolutionarily conserved murine serine threonine kinase, encodes multiple testis transcripts. | Shalom, S. and Don, J. 1999. Mol Reprod Dev. 52: 392-405. PMID: 10092119
- Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. | Silljé, HH., et al. 1999. EMBO J. 18: 5691-702. PMID: 10523312
- The Tousled gene in A. thaliana encodes a protein kinase homolog that is required for leaf and flower development. | Roe, JL., et al. 1993. Cell. 75: 939-50. PMID: 8252629
- TOUSLED is a nuclear serine/threonine protein kinase that requires a coiled-coil region for oligomerization and catalytic activity. | Roe, JL., et al. 1997. J Biol Chem. 272: 5838-45. PMID: 9038200
- TOUSLED participates in apical tissue formation during gynoecium development in Arabidopsis. | Roe, JL., et al. 1997. Plant Cell. 9: 335-53. PMID: 9090879
- cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal. | Yamakawa, A., et al. 1997. Gene. 202: 193-201. PMID: 9427565
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLK1 Antibody (G-8) | sc-393515 | 200 µg/ml | $316.00 | |||
TLK1 Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-530476 | 200 µg Ab; 10 µg BP | $354.00 | |||
TLK1 Antibody (G-8): m-IgGκ BP-HRP Bundle | sc-523974 | 200 µg Ab, 40 µg BP | $354.00 | |||
TLK1 Antibody (G-8): m-IgG2a BP-HRP Bundle | sc-547505 | 200 µg Ab; 10 µg BP | $354.00 | |||
TLK1 Antibody (G-8) AC | sc-393515 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TLK1 Antibody (G-8) HRP | sc-393515 HRP | 200 µg/ml | $316.00 | |||
TLK1 Antibody (G-8) FITC | sc-393515 FITC | 200 µg/ml | $330.00 | |||
TLK1 Antibody (G-8) PE | sc-393515 PE | 200 µg/ml | $343.00 | |||
TLK1 Antibody (G-8) Alexa Fluor® 488 | sc-393515 AF488 | 200 µg/ml | $357.00 | |||
TLK1 Antibody (G-8) Alexa Fluor® 546 | sc-393515 AF546 | 200 µg/ml | $357.00 | |||
TLK1 Antibody (G-8) Alexa Fluor® 594 | sc-393515 AF594 | 200 µg/ml | $357.00 | |||
TLK1 Antibody (G-8) Alexa Fluor® 647 | sc-393515 AF647 | 200 µg/ml | $357.00 | |||
TLK1 Antibody (G-8) Alexa Fluor® 680 | sc-393515 AF680 | 200 µg/ml | $357.00 | |||
TLK1 Antibody (G-8) Alexa Fluor® 790 | sc-393515 AF790 | 200 µg/ml | $357.00 | |||
TLK1 (G-8) Neutralizing Peptide | sc-393515 P | 100 µg/0.5 ml | $68.00 |