SAP 61 Activators
SAP 61 Activators primarily function through influencing phosphorylation cascades and altering transcription regulations. For instance, Phorbol 12-Myristate 13-Acetate (PMA) is a potent activator of Protein Kinase C (PKC), a family of protein kinases involved in controlling the function of other proteins through direct phosphorylation. This activation of PKC can enhance the phosphorylation of the SF3A3 complex, of which SAP 61 is a part, and therefore increase SAP 61's splicing activity. Similarly, Calyculin A and Okadaic acidwork by inhibiting protein phosphatases 1 and 2A, leading to increased phosphorylation events in cells. This action can stimulate the functional activity of SAP 61 by enhancing the phosphorylation of the SF3A3 spliceosomal complex.
Another group of SAP 61 activators, including Forskolin, Etoposide, and Anisomycin, function by altering cellular signaling pathways. For instance, Forskolin activates adenylyl cyclase, which indirectly affects the PKA pathway. This alteration in the PKA pathway can boost the functional activity of SAP 61 by enhancing the phosphorylation of spliceosomal components like the SF3A3 complex. Etoposide, on the other hand, works by initiating the DNA damage response pathway, which can lead to an increase in splicing activity. This DNA damage response can indirectly enhance the function of SAP 61, a key player in the RNA splicing process. Anisomycin, a JNK activator, enhances the phosphorylation of the SF3A3 complex, leading to increased functional activity of SAP 61.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA is a potent activator of Protein Kinase C (PKC), a family of protein kinases involved in controlling the function of other proteins through direct phosphorylation. Since SAP 61, as part of SF3A3 spliceosome complex, is involved in pre-mRNA splicing, the activation of PKC can lead to the enhanced phosphorylation of the SF3A3 complex and increase the splicing activity of SAP 61. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A inhibits protein phosphatases 1 and 2A, leading to an increase in the phosphorylation events in the cell. This can enhance the phosphorylation of the SF3A3 complex, in which SAP 61 serves as a subunit, and increase SAP 61's functional activity in the mRNA splicing process. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid inhibits protein phosphatases 1 and 2A. By inhibiting these phosphatases, it can increase the phosphorylation of the SF3A3 complex, enhancing the functional activity of SAP 61 in the mRNA splicing process. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin activates adenylyl cyclase, increasing the level of cAMP in the cell, which indirectly affects the PKA pathway. PKA can enhance the phosphorylation of spliceosomal components, including the SF3A3 complex, and increase the splicing activity of SAP 61. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $51.00 $231.00 $523.00 | 63 | |
Etoposide causes DNA damage and initiates the DNA damage response pathway, which often leads to an increase in splicing activity. Since SAP 61 is directly involved in the splicing process as a component of the SF3A3 complex, Etoposide can indirectly enhance its functional activity. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $153.00 $396.00 | 113 | |
Staurosporine is a broad-spectrum protein kinase inhibitor that can alter phosphorylation cascades involved in transcription regulation. This could potentially enhance the phosphorylation of the SF3A3 complex and thus the functional activity of SAP 61 in the mRNA splicing process. | ||||||
PD 98059 | 167869-21-8 | sc-3532 sc-3532A | 1 mg 5 mg | $40.00 $92.00 | 212 | |
PD98059 is an inhibitor of MEK, affecting the MAPK/ERK pathway. This compound can alter the phosphorylation state of the SF3A3 complex, leading to an increased functional activity of SAP 61 in the splicing process. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a PI3K inhibitor. By affecting the PI3K/Akt pathway, it can influence the phosphorylation state of the SF3A3 complex, leading to an increase in the functional activity of SAP 61 in the splicing process. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $90.00 $349.00 | 284 | |
SB203580 is an inhibitor of p38 MAPK. By inhibiting this pathway, it can alter the phosphorylation state of the SF3A3 complex, leading to an increase in the functional activity of SAP 61 in the splicing process. | ||||||
SP600125 | 129-56-6 | sc-200635 sc-200635A | 10 mg 50 mg | $40.00 $150.00 | 257 | |
SP600125 is an inhibitor of JNK. By inhibiting JNK, it can alter the phosphorylation state of the SF3A3 complex, leading to an increase in the functional activity of SAP 61 in the splicing process. | ||||||