Herc1 Activators
Herc1 Activators are a collection of chemical compounds that influence various intracellular signaling pathways, consequently enhancing the functional activity of Herc1, which is known for its role as an E3 ubiquitin ligase. Forskolin, by raising the levels of cAMP, indirectly promotes the activation of protein kinase A (PKA). This activation can lead to phosphorylation events that may affect the proteins Herc1 interacts with, potentially increasing Herc1's catalytic efficiency in tagging proteins with ubiquitin. Similarly, the phorbol ester PMA activates protein kinase C (PKC) which can phosphorylate proteins within the Herc1 signaling network, thus potentially enhancing Herc1's ligase activity by facilitating the ubiquitination process. Calcium signaling modifiers such as Ionomycin and Thapsigargin, by increasing intracellular calcium levels, can activate calcium-dependent proteases or other calcium-sensitive signaling mechanisms that may indirectly increase Herc1's activityby affecting substrate availability or conformation.
Continuing with other modulators, 8-Br-cAMP and db-cAMP, analogs of cAMP, also enhance PKA signaling, which can cause phosphorylation changes in Herc1's network, thereby indirectly amplifying Herc1's ubiquitination capacity. The inhibition of protein phosphatases by Okadaic acid and Calyculin A leads to an increase in the phosphorylated state of cellular proteins, some of which might be key to Herc1's ubiquitin ligase function, thus potentially enhancing Herc1's activity toward these substrates. Sphingosine-1-phosphate, as a lipid signaling molecule, could activate pathways that intersect with those regulated by Herc1, ultimately facilitating its role in ubiquitination. Likewise, LY294002, by inhibiting the PI3K/Akt pathway, and U0126 and SB203580, by inhibiting the MEK/ERK and p38 MAPK pathways respectively, can modify protein interactions and phosphorylation states that are crucial for Herc1's function.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylyl cyclase, increasing intracellular cAMP levels. Elevated cAMP activates PKA, which can phosphorylate various substrates including proteins that Herc1 may interact with, thereby potentially enhancing Herc1's E3 ubiquitin ligase activity through conformational changes or substrate targeting. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA is an activator of protein kinase C (PKC), which phosphorylates a wide array of target proteins. PKC-mediated phosphorylation of proteins within Herc1's pathway could lead to enhanced Herc1 functional activity related to protein ubiquitination. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium concentrations, potentially influencing calcium-dependent proteases that could alter the function or availability of Herc1's substrates, indirectly enhancing Herc1's activity. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $97.00 $224.00 | 30 | |
8-Br-cAMP is a cell-permeable cAMP analog that activates cAMP-dependent pathways, such as PKA signaling. This activation could lead to post-translational modifications of proteins within Herc1's network, indirectly enhancing Herc1's ubiquitination processes. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
db-cAMP is another cAMP analog that diffuses into cells and mimics the action of endogenous cAMP, activating PKA and potentially influencing Herc1 activity by modifying the phosphorylation state of proteins that interact with Herc1. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation levels within the cell. As phosphorylation status can influence Herc1's interaction with substrates, this compound could enhance Herc1's ubiquitin ligase activity. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A, like okadaic acid, is an inhibitor of protein phosphatases. This inhibition can lead to a buildup of phosphorylated proteins, some of which may be relevant to Herc1's function, indirectly enhancing its activity. | ||||||
D-erythro-Sphingosine-1-phosphate | 26993-30-6 | sc-201383 sc-201383D sc-201383A sc-201383B sc-201383C | 1 mg 2 mg 5 mg 10 mg 25 mg | $162.00 $316.00 $559.00 $889.00 $1693.00 | 7 | |
Sphingosine-1-phosphate is a signaling lipid that activates sphingosine-1-phosphate receptors, leading to downstream effects on cellular pathways that could intersect with Herc1's functional role, thereby enhancing its activity. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor that leads to increased cytosolic calcium levels, which could activate calcium-dependent signaling pathways, indirectly influencing Herc1's ligase activity through the modulation of its substrates. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is an inhibitor of PI3K, which affects the PI3K/Akt signaling pathway. Inhibition of this pathway can alter the phosphorylation status of various proteins, potentially modifying the activity of Herc1 by changing the ubiquitination landscape. | ||||||