CELF5 Inhibitors
Chemical inhibitors of CELF5 operate through various mechanisms to impede its function in RNA processing. Leptomycin B, for instance, targets exportin 1 (CRM1), a key protein in the nuclear export pathway. By inhibiting CRM1, Leptomycin B can cause the nuclear retention of CELF5, thereby preventing it from carrying out its typical roles in the cytoplasm. Similarly, Mitoxantrone, through its intercalation with DNA and inhibition of topoisomerase II, disrupts DNA synthesis and repair. This alteration in the nuclear environment can indirectly affect CELF5 by modifying post-transcriptional control mechanisms that require CELF5 activity. Another inhibitor, Alpha-amanitin, directly inhibits RNA polymerase II, crucial for mRNA synthesis, thereby reducing the availability of RNA substrates for CELF5, which in turn affects CELF5's RNA binding and processing activities. Actinomycin D also binds to DNA but does so at the transcription initiation complex, which prevents RNA chain elongation by RNA polymerase, resulting in a reduction in the RNA substrates needed for CELF5 function.
Further down the line of transcription-related inhibitors, DRB and Flavopiridol inhibit RNA polymerase II-mediated transcription, which can decrease the transcription of RNAs that CELF5 is meant to bind and regulate, thus inhibiting its function. Camptothecin and Etoposide, both topoisomerase inhibitors, cause DNA damage, which can disrupt transcription processes and subsequently hinder CELF5's interaction with RNA transcripts. Triptolide's broad inhibition of RNA polymerases I, II, and III and Aphidicolin's selective inhibition of DNA polymerases alpha and delta both lead to a general decrease in RNA synthesis, affecting the pool of RNA molecules available for CELF5 to act upon. Cordycepin, due to its structural similarity to adenosine, acts as a chain terminator during RNA synthesis, which reduces the length and quantity of RNA transcripts for CELF5 binding and regulation. Lastly, ICRF-193's stabilization of the topoisomerase II cleavable complex results in transcriptional inhibition, which can indirectly inhibit CELF5 by interfering with the maturation of RNA transcripts CELF5 typically regulates.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Leptomycin B | 87081-35-4 | sc-358688 sc-358688A sc-358688B | 50 µg 500 µg 2.5 mg | $107.00 $416.00 $1248.00 | 35 | |
Leptomycin B inhibits exportin 1 (CRM1), a nuclear export protein. CELF5, as a nuclear RNA-binding protein, may rely on properly functioning nuclear export signals for its localization and function. Inhibition of CRM1 can lead to nuclear retention of CELF5, thereby functionally inhibiting its normal cytoplasmic activities. | ||||||
Mitoxantrone | 65271-80-9 | sc-207888 | 100 mg | $285.00 | 8 | |
Mitoxantrone intercalates with DNA and inhibits topoisomerase II, disrupting DNA synthesis and repair. As CELF5 is associated with RNA processing events that can be influenced by DNA dynamics, mitoxantrone can indirectly inhibit CELF5 function by altering the nuclear environment and affecting the post-transcriptional control mechanisms where CELF5 is involved. | ||||||
α-Amanitin | 23109-05-9 | sc-202440 sc-202440A | 1 mg 5 mg | $269.00 $1050.00 | 26 | |
Alpha-amanitin is a potent inhibitor of RNA polymerase II, which is essential for mRNA synthesis. By inhibiting RNA polymerase II, alpha-amanitin can reduce the pool of RNA substrates available for CELF5, thereby functionally inhibiting its RNA binding and processing activities. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $74.00 $243.00 $731.00 $2572.00 $21848.00 | 53 | |
Actinomycin D binds to DNA at the transcription initiation complex and prevents elongation of RNA chains by RNA polymerase, which can reduce the RNA binding and modulation activity of CELF5 due to decreased RNA substrate availability. | ||||||
DRB | 53-85-0 | sc-200581 sc-200581A sc-200581B sc-200581C | 10 mg 50 mg 100 mg 250 mg | $43.00 $189.00 $316.00 $663.00 | 6 | |
DRB is an adenosine analog that inhibits RNA Polymerase II transcription. By reducing the overall transcription levels, DRB decreases the availability of RNA for CELF5 to bind, thus functionally inhibiting its role in RNA processing. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $58.00 $186.00 $94.00 | 21 | |
Camptothecin inhibits topoisomerase I, leading to DNA damage. CELF5's RNA processing functions are closely tied to the transcriptional machinery, and DNA damage can disrupt transcription processes, indirectly inhibiting CELF5's ability to interact with newly synthesized RNA transcripts. | ||||||
Triptolide | 38748-32-2 | sc-200122 sc-200122A | 1 mg 5 mg | $90.00 $204.00 | 13 | |
Triptolide is a diterpene triepoxide that inhibits the activity of RNA polymerase I, II, and III, leading to a reduction in RNA synthesis. This can functionally inhibit CELF5 by decreasing the pool of RNA molecules that CELF5 would typically bind and regulate. | ||||||
Flavopiridol | 146426-40-6 | sc-202157 sc-202157A | 5 mg 25 mg | $78.00 $259.00 | 41 | |
Flavopiridol is a cyclin-dependent kinase inhibitor that blocks RNA polymerase II-mediated transcription. This can functionally inhibit CELF5 by reducing the transcription of RNAs that CELF5 would act upon during RNA processing. | ||||||
ICRF-193 | 21416-68-2 | sc-200889 sc-200889A | 1 mg 5 mg | $341.00 $927.00 | 7 | |
ICRF-193 is a bis(dioxopiperazine) compound that stabilizes the cleavable complex of topoisomerase II, leading to transcriptional inhibition. This stabilization can indirectly inhibit CELF5 by hindering the processing and maturation of RNA transcripts to which CELF5 normally binds. | ||||||
Aphidicolin | 38966-21-1 | sc-201535 sc-201535A sc-201535B | 1 mg 5 mg 25 mg | $84.00 $306.00 $1104.00 | 30 | |
Aphidicolin is a tetracyclic diterpene that selectively inhibits DNA polymerase alpha and delta, leading to S-phase arrest in the cell cycle. This arrest can reduce the transcriptional output of the cell, indirectly inhibiting the functional role of CELF5 in RNA processing. | ||||||