CELF3 Activators
CELF3 activators encompass a diverse array of chemical compounds that indirectly enhance the functional activity of CELF3, primarily through the modulation of intracellular signaling pathways that are crucial for the regulation of alternative splicing. Forskolin, 8-Br-cAMP, and Isoproterenol all function by increasing intracellular cAMP levels, which in turn activate PKA. The activation of PKA is known to phosphorylate serine/arginine-rich (SR) proteins, which are critical co-regulators of splicing; this phosphorylation may enhance CELF3's capacity to regulate splicing by promoting its interaction with target pre-mRNAs and influencing splice siteselection. Similarly, Ionomycin and A23187, both calcium ionophores, as well as BAY K8644, an agonist of L-type calcium channels, elevate intracellular calcium levels, leading to the activation of calcium/calmodulin-dependent protein kinase (CaMK). CaMK's activity is linked to the phosphorylation of splicing factors, which could amplify CELF3's role in spliceosome assembly and function, thereby enhancing its regulatory influence on RNA splicing.
The remaining compounds on the list further contribute to the enhancement of CELF3's activity through various indirect mechanisms. Ro 20-1724, by inhibiting phosphodiesterase, and thus maintaining elevated cAMP levels, further supports PKA activity and consequent effects on splicing regulation by CELF3. Anisomycin's activation of JNK may modulate the splicing machinery, influencing CELF3's activity. KN-93, through its inhibitory action on CaMKII, may lead to compensatory activation of alternative pathways that synergize with CELF3's function. Thapsigargin, by disrupting calcium homeostasis, activates CaMK and could thereby potentiate CELF3's splicing regulatory capabilities. Lastly, 4-Phenylbutyrate (4-PBA) assists in maintaining cellular homeostasis, which is foundational for the optimal functioning of the splicing machinery, thereby indirectly supporting CELF3's role in alternative splicing processes. Collectively, these activators enhance the functional activity of CELF3 by modulating the phosphorylation state and activity of splicing factors and maintaining cellular environments conducive to effective splicing regulation.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin activates adenylyl cyclase, which increases intracellular cAMP levels. Elevated cAMP activates PKA, which can phosphorylate serine/arginine-rich (SR) proteins, potentially enhancing the splicing activity of CELF3 by promoting its interaction with target pre-mRNAs. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $126.00 $328.00 | 30 | |
8-Br-cAMP is a cAMP analogue that activates PKA. Activated PKA may enhance phosphorylation of factors in the splicing machinery, thus potentially increasing the splicing regulatory activity of CELF3. | ||||||
Ionomycin, free acid | 56092-81-0 | sc-263405 sc-263405A | 1 mg 5 mg | $96.00 $264.00 | 2 | |
Ionomycin is a calcium ionophore that increases intracellular Ca2+ concentration, which can activate calcium/calmodulin-dependent protein kinase (CaMK). CaMK can phosphorylate splicing factors, possibly augmenting the RNA binding and splicing regulatory functions of CELF3. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $28.00 $38.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that can elevate intracellular cAMP levels, thereby activating PKA. PKA may indirectly enhance the functional activity of CELF3 by phosphorylation of associated splicing factors. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which can phosphorylate and regulate splicing factors involved in the regulation of alternative splicing, potentially enhancing the activity of CELF3 in the splicing process. | ||||||
(±)-Bay K 8644 | 71145-03-4 | sc-203324 sc-203324A sc-203324B | 1 mg 5 mg 50 mg | $84.00 $196.00 $817.00 | ||
BAY K8644 is an L-type calcium channel agonist that increases Ca2+ influx, which can activate CaMK. The activation of CaMK may lead to an enhancement of CELF3 activity through phosphorylation of SR proteins and other splicing factors. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 is another calcium ionophore, which, like Ionomycin, can elevate intracellular calcium levels and activate CaMK-dependent signaling pathways, potentially increasing the splicing regulation function of CELF3. | ||||||
Ro 20-1724 | 29925-17-5 | sc-200709 sc-200709A sc-200709B | 100 mg 1 g 5 g | $87.00 $426.00 $1574.00 | 17 | |
Ro 20-1724 inhibits phosphodiesterase (PDE), leading to increased cAMP levels. This elevation of cAMP could enhance PKA activity, which may indirectly increase CELF3's splicing activity by phosphorylating associated factors. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a JNK activator that can modulate the activity of splicing factors through phosphorylation. This modulation may influence alternative splicing events controlled by CELF3. | ||||||
KN-93 | 139298-40-1 | sc-202199 | 1 mg | $182.00 | 25 | |
KN-93 is an inhibitor of CaMKII which can, paradoxically, enhance the function of CELF3 by modulating the activity of SR proteins, as the inhibition of CaMKII may lead to compensatory mechanisms activating other splicing factors that work in tandem with CELF3. | ||||||