Cdc45 Activators
Cdc45, a crucial protein in eukaryotic DNA replication, plays a central role in the initiation and progression of DNA replication forks. Its primary function is to facilitate the assembly of the pre-initiation complex (pre-IC) at origins of DNA replication and to recruit additional replication factors essential for DNA synthesis. During the initiation phase of DNA replication, Cdc45 is recruited to replication origins in association with other key proteins, including the origin recognition complex (ORC), Cdc6, and the minichromosome maintenance (MCM) complex. This recruitment is essential for the formation of the pre-IC, which serves as the platform for the assembly of the DNA replication machinery. Additionally, Cdc45 interacts with DNA polymerases and helicases, contributing to the unwinding of DNA strands and the establishment of replication forks.
Activation of Cdc45 is tightly regulated to ensure accurate and efficient DNA replication. The initiation of DNA replication and subsequent recruitment of Cdc45 to replication origins are orchestrated by a series of regulatory events. One critical step in Cdc45 activation involves the phosphorylation of key residues by cyclin-dependent kinases (CDKs) and Dbf4-dependent kinase (DDK). Phosphorylation of Cdc45 by these kinases promotes its association with the MCM complex and facilitates the formation of functional replication forks. Additionally, the binding of Cdc45 to other replication factors, such as the GINS complex, further stabilizes its interaction with DNA and promotes replication fork progression. Moreover, the temporal regulation of Cdc45 expression and localization throughout the cell cycle ensures proper coordination of DNA replication processes. Overall, elucidating the mechanisms of Cdc45 activation provides valuable insights into the regulation of DNA replication and genome stability, with implications for understanding cellular proliferation and the development of novel strategies for targeting DNA replication in various biological contexts.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog. It activates Protein Kinase A (PKA) by mimicking cAMP. PKA can phosphorylate various targets involved in cell cycle regulation. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a PI3K inhibitor. By inhibiting PI3K, it can lead to a compensatory upregulation of other pathways that might enhance cell cycle progression. In certain contexts, this compensatory mechanism might increase the activity of factors involved in DNA replication, indirectly facilitating the role of Cdc45 in the replication process. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $62.00 $155.00 $320.00 | 233 | |
Rapamycin is an mTOR inhibitor. Inhibition of mTOR can lead to altered cellular responses, including changes in cell cycle progression. In certain cells, this might indirectly upregulate the factors involved in DNA replication, thereby promoting Cdc45 activity as it plays a crucial role in the initiation of DNA replication. | ||||||
PD 98059 | 167869-21-8 | sc-3532 sc-3532A | 1 mg 5 mg | $39.00 $90.00 | 212 | |
PD98059 is a MEK inhibitor. By inhibiting MEK, it can lead to compensatory cellular responses that might enhance the expression or function of factors involved in DNA replication. This indirect effect can potentially promote the activity of Cdc45, which is essential for the DNA replication process. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $88.00 $342.00 | 284 | |
SB203580 is a p38 MAPK inhibitor. Inhibition of p38 MAPK can lead to altered cellular responses, including changes in cell cycle regulation. This might indirectly enhance the expression or activity of factors involved in DNA replication, thereby potentially promoting Cdc45 activity. | ||||||
SP600125 | 129-56-6 | sc-200635 sc-200635A | 10 mg 50 mg | $40.00 $150.00 | 257 | |
SP600125 is a JNK inhibitor. By inhibiting JNK, it may induce compensatory cellular responses that enhance cell cycle progression. This could indirectly result in the upregulation of factors necessary for DNA replication, potentially facilitating Cdc45's role in this process. | ||||||
Roscovitine | 186692-46-6 | sc-24002 sc-24002A | 1 mg 5 mg | $92.00 $260.00 | 42 | |
Roscovitine is a cyclin-dependent kinase (CDK) inhibitor, primarily targeting CDK2, CDK7, and CDK9. While it inhibits CDKs, paradoxically, in some contexts, it can cause a compensatory upregulation of other cell cycle proteins, potentially indirectly enhancing the activity of Cdc45 in the DNA replication process. | ||||||
Kenpaullone | 142273-20-9 | sc-200643 sc-200643A sc-200643B sc-200643C | 1 mg 5 mg 10 mg 25 mg | $60.00 $150.00 $226.00 $495.00 | 1 | |
Kenpaullone is another CDK inhibitor with a broad target range, including GSK-3β. By inhibiting these kinases, Kenpaullone might induce compensatory mechanisms in the cell that lead to upregulation of factors involved in cell cycle progression and DNA replication, potentially promoting Cdc45 activity. | ||||||
Thiazovivin | 1226056-71-8 | sc-361380 sc-361380A | 10 mg 25 mg | $278.00 $622.00 | 15 | |
Thiazovivin is a ROCK inhibitor, known for enhancing cell survival and proliferation. By inhibiting ROCK, it may influence cell cycle progression indirectly. This can lead to an upregulation of factors necessary for DNA replication, thereby potentially enhancing the activity of Cdc45. | ||||||
ZM-447439 | 331771-20-1 | sc-200696 sc-200696A | 1 mg 10 mg | $150.00 $349.00 | 15 | |
ZM-447439 is an Aurora kinase inhibitor. While its primary action is inhibition, it can cause complex changes in cell cycle regulation. These changes might indirectly result in the upregulation of factors involved in DNA replication, potentially promoting Cdc45 activity. | ||||||